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Literature summary extracted from
- A conserved tyrosine residue of Saccharomyces cerevisiae leukotriene A4 hydrolase stabilizes the transition state of the peptidase activity (2006), Peptides, 27, 1701-1709.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.3.2.6 | into the pYES2.1-Topo vector for expression of the recombinant proteins in Saccharomyces cerevisiae 1971 | Saccharomyces cerevisiae |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 3.3.2.6 | Y244F | leukotriene A4 hydrolase mutant | Saccharomyces cerevisiae |
| 3.3.2.6 | Y256F | leukotriene A4 hydrolase mutant | Saccharomyces cerevisiae |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.3.2.6 | bestatin | - |
Saccharomyces cerevisiae |  |
| 3.3.2.6 | puromycin | - |
Saccharomyces cerevisiae | |
| 3.3.2.6 | RB-3014 | - |
Saccharomyces cerevisiae | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.3.2.6 | Zn2+ | - |
Saccharomyces cerevisiae | |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.3.2.6 | (7E,9E,11Z,14Z)-(5S,6S)-5,6-epoxyicosa-7,9,11,14-tetraenoate + H2O | Saccharomyces cerevisiae | substrate for epoxide hydrolase activity | (6Z,8E,10E,14Z)-(5S,12R)-5,12-dihydroxyicosa-6,8,10,14-tetraenoate | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.3.2.6 | Saccharomyces cerevisiae | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.3.2.6 | using metal affinity chromatography, the His tag is removed by digestion with His6-TEV protease | Saccharomyces cerevisiae |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.3.2.6 | (7E,9E,11Z,14Z)-(5S,6S)-5,6-epoxyicosa-7,9,11,14-tetraenoate + H2O | substrate for epoxide hydrolase activity | Saccharomyces cerevisiae | (6Z,8E,10E,14Z)-(5S,12R)-5,12-dihydroxyicosa-6,8,10,14-tetraenoate | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.3.2.6 | leukotriene-A4 hydrolase | - |
Saccharomyces cerevisiae |
| 3.3.2.6 | LTA4H | - |
Saccharomyces cerevisiae |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.3.2.6 | 20 | - |
activity assay | Saccharomyces cerevisiae |
Ki Value [mM]
| EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.3.2.6 | 0.000087 | - |
RB-3014 | wild-type LTA4H | Saccharomyces cerevisiae | |
| 3.3.2.6 | 0.00074 | - |
RB-3014 | mutant Y456F | Saccharomyces cerevisiae | |
| 3.3.2.6 | 0.0027 | - |
bestatin | wild-type LTA4H | Saccharomyces cerevisiae | |
| 3.3.2.6 | 0.0037 | - |
RB-3014 | mutant Y244F | Saccharomyces cerevisiae | |
| 3.3.2.6 | 0.049 | - |
bestatin | mutant Y456F | Saccharomyces cerevisiae | |
| 3.3.2.6 | 0.0765 | - |
bestatin | mutant Y244F | Saccharomyces cerevisiae | |
| 3.3.2.6 | 2.37 | - |
puromycin | mutant Y244F | Saccharomyces cerevisiae | |
| 3.3.2.6 | 3.16 | - |
puromycin | wild-type LTA4H | Saccharomyces cerevisiae | |
| 3.3.2.6 | 3.24 | - |
puromycin | mutant Y456F | Saccharomyces cerevisiae | |
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Release 2023.1 (January 2023)
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