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Literature summary extracted from

  • Liu, G.; Nakayama, K.; Awata, S.; Tang, S.; Kitaoka, N.; Manabe, M.; Kodama, H.
    Prolidase isoenzymes in the rat: their organ distribution, developmental change and specific inhibitors (2007), Pediatr. Res., 62, 54-59.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.13.9 captopril inhibits isozyme PD I dose-dependently, but shows no inhibition of isozyme PD II at 0.1 mM Rattus norvegicus
3.4.13.9 N-Benzyloxycarbonyl-L-proline inhibits isozyme PD I dose-dependently, but shows no inhibition of isozyme PD II at up to 1 mM Rattus norvegicus
3.4.13.9 Ni2+
-
 Rattus norvegicus
3.4.13.9 NiCl2 inhibits isozye PD II much more effectively than isozyme PD I Rattus norvegicus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.13.9 Mn2+ required Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.13.9 additional information Rattus norvegicus lack of prolidase I leads to prolidase deficiency, a disease characterized by intractable skin lesions, recurrent respiratory infections, and mental retardation, physiologic roles of prolidase isoenzymes, PD I functions by way of an intestinal peptide carrier, which may be regulated by the uptake of various iminodipeptides, intestinal PD II also participates in absorption of proline and other amino acids early in life, overview ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.13.9 Rattus norvegicus
-
 male Wistar rats, isozymes PD I and PD II
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.13.9 native isozymes partially from kidney by anion exchange chromatography Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.13.9 brain
-
 Rattus norvegicus
-
3.4.13.9 heart
-
 Rattus norvegicus
-
3.4.13.9 kidney isozymes PD I and PD II, high expression level of isozyme PD I, which is predominantly concentrated in renal cortex, where its activity is 2fold higher than in renal medulla Rattus norvegicus
-
3.4.13.9 liver
-
 Rattus norvegicus
-
3.4.13.9 lung
-
 Rattus norvegicus
-
3.4.13.9 additional information organ distribution and developmental changes of isozymes PD I and PD II, overview Rattus norvegicus
-
3.4.13.9 pancreas
-
 Rattus norvegicus
-
3.4.13.9 small intestine high expression level of isozyme PD I Rattus norvegicus
-
3.4.13.9 spleen
-
 Rattus norvegicus
-
3.4.13.9 stomach
-
 Rattus norvegicus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.13.9 Ala-Pro + H2O preferred substrate of isozyme PD I Rattus norvegicus Ala + Pro
-
 ?
3.4.13.9 Gly-Pro + H2O
-
 Rattus norvegicus Gly + Pro
-
 ?
3.4.13.9 Met-Pro + H2O preferred substrate of isozyme PD II Rattus norvegicus Met + Pro
-
 ?
3.4.13.9 additional information substrate specificity of isozymes, overview Rattus norvegicus ?
-
 ?
3.4.13.9 additional information lack of prolidase I leads to prolidase deficiency, a disease characterized by intractable skin lesions, recurrent respiratory infections, and mental retardation, physiologic roles of prolidase isoenzymes, PD I functions by way of an intestinal peptide carrier, which may be regulated by the uptake of various iminodipeptides, intestinal PD II also participates in absorption of proline and other amino acids early in life, overview Rattus norvegicus ?
-
 ?
3.4.13.9 Ser-Pro + H2O preferred substrate of isozyme PD I Rattus norvegicus Ser + Pro
-
 ?
3.4.13.9 Val-Pro + H2O
-
 Rattus norvegicus Val + Pro
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.13.9 prolidase I
-
 Rattus norvegicus
3.4.13.9 prolidase II
-
 Rattus norvegicus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.13.9 37
-
 assay at Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.13.9 7.8
-
 assay at Rattus norvegicus

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
3.4.13.9 0.005
-
 isozyme PD I, pH 7.8, 37°C Rattus norvegicus N-Benzyloxycarbonyl-L-proline
3.4.13.9 0.05
-
 isozyme PD I, pH 7.8, 37°C Rattus norvegicus captopril
3.4.13.9 0.05
-
 isozyme PD II, pH 7.8, 37°C Rattus norvegicus Ni2+