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Literature summary extracted from
- Restriction endonuclease BpuJI specific for the 5-CCCGT sequence is related to the archaeal Holliday junction resolvase family (2007), Nucleic Acids Res., 35, 2377-2389.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.1.21.10 | for expression in Escherichia coli ER2267 cells | Bacillus pumilus |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.21.10 | Mg2+ | BpuJI requires Mg2+-ions for DNA cleavage | Bacillus pumilus |  |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.1.21.10 | 53900 | - |
predicted molecular mass of the BpuJI subunit | Bacillus pumilus |
| 3.1.21.10 | 109000 | - |
homodimer, determined by sedimentation equilibrium experiments using an analytical ultracentrifuge | Bacillus pumilus |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.1.21.10 | Bacillus pumilus | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.1.21.10 | using heparin-Sepharose, blue-Sepharose and AH-Sepharose columns | Bacillus pumilus |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.1.21.10 | homodimer | - |
Bacillus pumilus |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.1.21.10 | BpuJI | BpuJI is cleaved into a N-terminal domain, NTD, binding domain, and a C-terminal domain, CTD, catalytic domain, CTD is structurally related to archaeal Holliday junction resolvases | Bacillus pumilus |
| 3.1.21.10 | Holliday junction resolvase | - |
Bacillus pumilus |
| 3.1.21.10 | REase | - |
Bacillus pumilus |
| 3.1.21.10 | restriction endonuclease | - |
Bacillus pumilus |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.1.21.10 | 25 | - |
PCR fragment cleavage assay | Bacillus pumilus |
| 3.1.21.10 | 25 | - |
plasmid and lambda DNA cleavage assay | Bacillus pumilus |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.21.10 | 7.5 | - |
PCR fragment cleavage assay | Bacillus pumilus |
| 3.1.21.10 | 7.5 | - |
plasmid and lambda DNA cleavage assay | Bacillus pumilus |
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Release 2023.1 (January 2023)
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