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Literature summary extracted from

  • Kaiserman, D.; Bird, C.H.; Sun, J.; Matthews, A.; Ung, K.; Whisstock, J.C.; Thompson, P.E.; Trapani, J.A.; Bird, P.I.
    The major human and mouse granzymes are structurally and functionally divergent (2006), J. Cell Biol., 175, 619-630.
    View publication on PubMedView publication on EuropePMC

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.78 Homo sapiens P12544
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3.4.21.78 Mus musculus P11032
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Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.21.78 HeLa cell 51Cr-release assay applied Homo sapiens
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3.4.21.78 JURKAT cell standard MTT-assay applied Homo sapiens
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3.4.21.78 lymphoma cell standard MTT-assay applied Mus musculus
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Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.21.78 additional information
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 ability of human and mouse granzyme A to kill human or mouse cells in the presence of perforin analyzed by standard MTT-assay and by 51Cr-release assay, recombinant and native proteins tested, cytotoxic potential between mouse and human granzyme A different Homo sapiens
3.4.21.78 additional information
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 ability of human and mouse granzyme A to kill human or mouse cells in the presence of perforin analyzed, recombinant and native proteins tested, cytotoxic potential between mouse and human granzyme A different Homo sapiens
3.4.21.78 additional information
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 ability of human and mouse granzyme A to kill human or mouse cells in the presence of perforin analyzed, recombinant and native proteins tested, cytotoxic potential between mouse and human granzyme A different, mouse but not human granzyme A induces perforin-dependent cell death Mus musculus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.21.78 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide + H2O MTT, benzyloxy-carbonyl-Lys-thiobenzyl ester, standard MTT-assay measuring loss of mitochondrial respiration, interspecies differences of granzyme A between human and mouse analyzed, different cytotoxic potential identified Homo sapiens ?
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 ?
3.4.21.78 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide + H2O MTT, benzyloxy-carbonyl-Lys-thiobenzyl ester, standard MTT-assay measuring loss of mitochondrial respiration, interspecies differences of granzyme A between human and mouse analyzed, mouse granzyme A is considerably more cytotoxic than human granzyme A Mus musculus ?
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 ?
3.4.21.78 additional information interspecies differences of granzyme A between human and mouse analyzed by 51Cr-release assay, different cytotoxic potential identified Homo sapiens ?
-
 ?