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Literature summary extracted from

  • Izaguirre, G.; Swanson, R.; Raja, S.M.; Rezaie, A.R.; Olson, S.T.
    Mechanism by which exosites promote the inhibition of blood coagulation proteases by heparin-activated antithrombin (2007), J. Biol. Chem., 282, 33609-33622.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
3.4.21.6 S195A catalytically inactive mutant, that still binds to inhibitor antithrombin with the same velocity as the wild-type enzyme Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.21.6 antithrombin human recombinant protein, competitive inhibition, heparin-activated antithrombin, interaction and inhibition mechanism involving inhibitor residues Tyr253, as well as Glu255, Glu237, and Arg399, and exosites generated via heparin binding, rapid kinetic studies, wild-type and mutant antithrombins, overview Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.6 Homo sapiens
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.21.6 tosyl-Gly-Pro-Arg-7-amido-4-methylcoumarin + H2O
-
 Homo sapiens tosyl-Gly-Pro-Arg + 7-amino-4-methylcoumarin
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.21.6 25
-
 assay at Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.21.6 7.4
-
 assay at Homo sapiens

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.4.21.6 additional information
-
 additional information rapid inhibition kinetic studies, wild-type and mutant antithrombins, overview Homo sapiens