Literature summary extracted from
Preston, R.J.; Ajzner, E.; Razzari, C.; Karageorgi, S.; Dua, S.; Dahlbaeck, B.; Lane, D.A.
Multifunctional specificity of the protein C/activated protein C Gla domain (2006), J. Biol. Chem., 281, 28850-28857.
Activating Compound
EC Number |
Activating Compound |
Comment |
Organism |
Structure |
---|
3.4.21.69 |
phosphatidylcholine |
as phospholipid vesicles with 80% phosphatidylcholine and 20% phosphatidylserine, specific binding to the enzyme of anionic phospholipid vesicles passed over the protein C surface, defective phospholipid binding of mutant variants also resulted in severely impaired anticoagulant activity in protein C-deficient plasma |
Homo sapiens |
|
3.4.21.69 |
phosphatidylserine |
as phospholipid vesicles with 80% phosphatidylcholine and 20% phosphatidylserine, specific binding to the enzyme of anionic phospholipid vesicles passed over the protein C surface, defective phospholipid binding of mutant variants also resulted in severely impaired anticoagulant activity in protein C-deficient plasma |
Homo sapiens |
|
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
3.4.21.69 |
expression of wild-type and mutant enzymes in HEK-293 cells, secretion to the medium |
Homo sapiens |
Protein Variants
EC Number |
Protein Variants |
Comment |
Organism |
---|
3.4.21.69 |
D35T/D36A |
site-directed mutagenesis, the mutant shows slightly increased factor Va proteolysis compared to the wild-type enzyme, the activation by protein S is reduced |
Homo sapiens |
3.4.21.69 |
D36A/L38D/A39V |
site-directed mutagenesis, the mutant shows no activation by protein S |
Homo sapiens |
3.4.21.69 |
E16D |
site-directed mutagenesis, the mutation causes aberrant Ca2+ binding and Gla domain misfolding |
Homo sapiens |
3.4.21.69 |
N33S/V34S/D35T/D36A/L38D/A39V |
site-directed mutagenesis, the mutant shows slightly increased factor Va proteolysis compared to the wild-type enzyme, no activation by protein S |
Homo sapiens |
3.4.21.69 |
S11G/S12N |
site-directed mutagenesis, the mutant shows slightly increased factor Va proteolysis compared to the wild-type enzyme |
Homo sapiens |
Metals/Ions
EC Number |
Metals/Ions |
Comment |
Organism |
Structure |
---|
3.4.21.69 |
Ca2+ |
required |
Homo sapiens |
|
Natural Substrates/ Products (Substrates)
EC Number |
Natural Substrates |
Organism |
Comment (Nat. Sub.) |
Natural Products |
Comment (Nat. Pro.) |
Rev. |
Reac. |
---|
3.4.21.69 |
additional information |
Homo sapiens |
activated protein C has potent anticoagulant and antiinflammatory properties that are mediated in part by its interactions with its cofactor protein S and the endothelial cell protein C receptor, EPCR, the protein C/APC Gla domain is implicated in both interactions |
? |
- |
? |
|
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
3.4.21.69 |
Homo sapiens |
- |
- |
- |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
3.4.21.69 |
recombinant wild-type and mutant enzymes from HEK-293 cell culture medium by anion exchange chromatography |
Homo sapiens |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
3.4.21.69 |
active factor Va + H2O |
proteolytic inactivation |
Homo sapiens |
inactive factor V + domain B |
- |
? |
|
3.4.21.69 |
additional information |
activated protein C has potent anticoagulant and antiinflammatory properties that are mediated in part by its interactions with its cofactor protein S and the endothelial cell protein C receptor, EPCR, the protein C/APC Gla domain is implicated in both interactions |
Homo sapiens |
? |
- |
? |
|
3.4.21.69 |
S-2238 + H2O |
a chromogenic substrate |
Homo sapiens |
? |
- |
? |
|
Synonyms
EC Number |
Synonyms |
Comment |
Organism |
---|
3.4.21.69 |
Activated protein C |
- |
Homo sapiens |
3.4.21.69 |
APC |
- |
Homo sapiens |
Temperature Optimum [°C]
EC Number |
Temperature Optimum [°C] |
Temperature Optimum Maximum [°C] |
Comment |
Organism |
---|
3.4.21.69 |
37 |
- |
assay at |
Homo sapiens |
pH Optimum
EC Number |
pH Optimum Minimum |
pH Optimum Maximum |
Comment |
Organism |
---|
3.4.21.69 |
7.4 |
- |
assay at |
Homo sapiens |
Cofactor
EC Number |
Cofactor |
Comment |
Organism |
Structure |
---|
3.4.21.69 |
protein S |
required, activates, the position of APC Gla domain residues is critical for protein S enhancement of APC activity and EPCR recognition, binding structure, overview |
Homo sapiens |
|