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Literature summary extracted from

  • Neuenschwander, P.F.; Williamson, S.R.; Nalian, A.; Baker-Deadmond, K.J.
    Heparin modulates the 99-loop of factor IXa: effects on reactivity with isolated Kunitz-type inhibitor domains (2006), J. Biol. Chem., 281, 23066-23074.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.4.21.22 anionic phospholipid highly activating Homo sapiens
3.4.21.22 vitamin K dependent on Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.21.22 expression of wild-type and mutant enzymes in HEK-293 cells Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
3.4.21.22 K98A site-directed mutagenesis, Homo sapiens
3.4.21.22 additional information the modulatory effect of heparin can be completely abrogated by mutating a single amino acid residue in the 99-loop region of the extended fIXa active site cleft outside of the heparin binding exosite, overview Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.21.22 antithrombin in vivo and in vitro inhibition, the interaction is improved by heparin binding via the formation of bridged complexes where heparin acts as a template Homo sapiens
3.4.21.22 BPTI Kunitz-type inhibitor, competitive, reversible inhibition, low molecular weight heparin binding to fIXa enhances reactivity of fIXa with the Kunitz-type inhibitor BPTI Homo sapiens
3.4.21.22 enoxaparin competitive, reversible inhibition Homo sapiens
3.4.21.22 additional information oligosaccharide binding at loop 99 can allosterically modulate the fIXa active site region, overview Homo sapiens
3.4.21.22 PN2-KPI Kunitz-type inhibitor, competitive, reversible inhibition Homo sapiens
3.4.21.22 TFPI-K1 Kunitz-type inhibitor, competitive, reversible inhibition Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.21.22 extracellular
-
 Homo sapiens
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.21.22 Ca2+ hughly activating Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.21.22 factor X + H2O Homo sapiens factor IXa is a vitamin K-dependent blood coagulation factor that is essential for the amplification or consolidation phase of blood coagulation activated factor X + ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.22 Homo sapiens P00740
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.4.21.22 proteolytic modification activation by purified factor X activator from the venom of Russell’s Vipe cleaving fIX after Arg180 to generate active enzyme fIXaalpha Homo sapiens

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.21.22 recombinant wild-type and mutant enzymes from HEK-293 cells by anion exchange and heparin affinity chromatography Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.21.22 commercial preparation from plasma Homo sapiens
-
3.4.21.22 plasma
-
 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.21.22 CH3SO2-D-Leu-Gly-Arg-4-nitroanilide + H2O commercial chromogenic substrate CBS 31.39 Homo sapiens ?
-
 ?
3.4.21.22 factor X + H2O factor IXa is a vitamin K-dependent blood coagulation factor that is essential for the amplification or consolidation phase of blood coagulation Homo sapiens activated factor X + ?
-
 ?
3.4.21.22 factor X + H2O oligosaccharide binding at loop 99 can allosterically modulate the fIXa active site region Homo sapiens activated factor X + ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.21.22 factor IXa
-
 Homo sapiens
3.4.21.22 FIXa
-
 Homo sapiens
3.4.21.22 More the enzyme is a member of the serine protease family and shares a high degree of homology with trypsin Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.21.22 25
-
 assay at Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.21.22 8
-
 assay at Homo sapiens

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.4.21.22 additional information
-
 additional information inhibition kinetics Homo sapiens