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Literature summary extracted from

  • Teufel, M.; Saudek, V.; Ledig, J.P.; Bernhardt, A.; Boularand, S.; Carreau, A.; Cairns, N.J.; Carter, C.; Cowley, D.J.; Duverger, D.; Ganzhorn, A.J.; Guenet, C.; Heintzelmann, B.; Laucher, V.; Sauvage, C.; Smirnova, T.
    Sequence identification and characterization of human carnosinase and a closely related non-specific dipeptidase (2002), J. Biol. Chem., 278, 6521-31.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.13.20 expression in CHO cell Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
3.4.13.20 D166A mutation in putative metal binding site, complete loss of activity Homo sapiens
3.4.13.20 E201A mutation in putative metal binding site, complete loss of activity Homo sapiens
3.4.13.20 E201A/D229A mutation in putative metal binding site, complete loss of activity Homo sapiens
3.4.13.20 H133A mutation in putative metal binding site, complete loss of activity Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.13.20 bestatin
-
 Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.13.20 0.2
-
 homocarnosine pH 7.5, 30°C Homo sapiens
3.4.13.20 1.27
-
 beta-Ala-L-His pH 7.5, 30°C Homo sapiens
3.4.13.20 1.9
-
 homocarnosine presence of CD2+, pH 7.5, 30°C Homo sapiens
3.4.13.20 11
-
 beta-Ala-L-His presence of CD2+, pH 7.5, 30°C Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.13.20 extracellular
-
 Homo sapiens
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.13.20 Cd2+ increase in kcat value Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.13.20 52800
-
 2 * 82000, SDS-PAGE, 2 * 63700, MALDI-TOF, 2 * 52800, calculated Homo sapiens
3.4.13.20 63700
-
 2 * 82000, SDS-PAGE, 2 * 63700, MALDI-TOF, 2 * 52800, calculated Homo sapiens
3.4.13.20 82000
-
 2 * 82000, SDS-PAGE, 2 * 63700, MALDI-TOF, 2 * 52800, calculated Homo sapiens
3.4.13.20 167000
-
 gel filtration Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
3.4.13.20 Homo sapiens Q96KN2
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.4.13.20 glycoprotein after treatment with PNGase F, molecular mass shifts from 82000 Da to 62000 Da, SDS-PAGE Homo sapiens

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.13.20 recombinant enzyme Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.13.20 brain of adult. No expression in fetal brain Homo sapiens
-
3.4.13.20 central nervous system
-
 Homo sapiens
-
3.4.13.20 liver low expresion Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.13.20 beta-Ala-L-His + H2O
-
 Homo sapiens beta-Ala + L-His
-
 ?
3.4.13.20 Gly-L-His + H2O
-
 Homo sapiens Gly + L-His
-
 ?
3.4.13.20 homocarnosine + H2O
-
 Homo sapiens ?
-
 ?
3.4.13.20 L-Ala-L-His + H2O
-
 Homo sapiens L-Ala + L-His
-
 ?
3.4.13.20 additional information non Xaa-His dipeptides like Ala-Ala, or Ala-Pro, as well as tripeptides containing histidine in central or C-terminal position such as Gly-His-Gly or Gly-Gly-His, are not degraded Homo sapiens ?
-
 ?
3.4.13.20 N-methylcarnosine + H2O
-
 Homo sapiens N-methyl-beta-Ala + L-His
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.13.20 dimer 2 * 82000, SDS-PAGE, 2 * 63700, MALDI-TOF, 2 * 52800, calculated Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
3.4.13.20 CN1
-
 Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.13.20 0.2
-
 homocarnosine pH 7.5, 30°C Homo sapiens
3.4.13.20 10.6
-
 beta-Ala-L-His pH 7.5, 30°C Homo sapiens
3.4.13.20 18
-
 homocarnosine presence of CD2+, pH 7.5, 30°C Homo sapiens
3.4.13.20 138
-
 beta-Ala-L-His presence of CD2+, pH 7.5, 30°C Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.13.20 7.5 8.5 broad Homo sapiens

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.4.13.20 0.000007
-
 bestatin pH 7.5, 30°C Homo sapiens

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.4.13.20 Homo sapiens calculated
-
 4.5