EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.4.24.27 | Co2+ | competitive, 72% inhibition at 18 mM, partial protection or reverse effect by Ca2+ at 0.5 mM, Co2+ show partly Ca2+-sensitive and partly Ca2+-insensitive inhibition, molecular mechanism, Co21 accelerates the autolysis, overview | Bacillus thermoproteolyticus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.4.24.27 | additional information | - |
additional information | kinetics | Bacillus thermoproteolyticus |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.4.24.27 | Ca2+ | four ions per enzyme molecule required for stability | Bacillus thermoproteolyticus | |
3.4.24.27 | Zn2+ | one ion per enzyme molecule required for activity | Bacillus thermoproteolyticus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.4.24.27 | Bacillus thermoproteolyticus | - |
- |
- |
EC Number | Posttranslational Modification | Comment | Organism |
---|---|---|---|
3.4.24.27 | proteolytic modification | thermolysin performs Co2+-stimulable autolysis | Bacillus thermoproteolyticus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.4.24.27 | additional information | thermolysin performs Co2+-stimulable autolysis | Bacillus thermoproteolyticus | ? | - |
? | |
3.4.24.27 | N-[3-(2-furyl)acryloyl]-glycyl-L-leucine amide + H2O | i.e. FAGLA | Bacillus thermoproteolyticus | ? | - |
? | |
3.4.24.27 | N-[3-(2-furyl)acryloyl]-L-leucine-L-alanine amide + H2O | i.e. FALAA | Bacillus thermoproteolyticus | ? | - |
? |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.4.24.27 | 25 | - |
assay at | Bacillus thermoproteolyticus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.4.24.27 | 7 | - |
- |
Bacillus thermoproteolyticus |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.4.24.27 | additional information | - |
additional information | inhibition kinetics with Co2+ and Ca2+, overview | Bacillus thermoproteolyticus |