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Literature summary extracted from

  • Crippa, M.P.
    Urokinase-type plasminogen activator (2007), Int. J. Biochem. Cell Biol., 39, 690-694.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.21.73 DNA and amino acid sequence analysis Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.21.73 additional information the active form of uPA is bound to its high affinity receptor on the cell surface, where specific inhibitors modulate its enzymatic activity, such inhibitors also regulate the cell surface levels of uPA by triggering the internalization of the uPA-receptor-inhibitor complex via endocytosis Homo sapiens
3.4.21.73 plasminogen activator inhibitor 1
-
 Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.21.73 cell surface the active form of uPA is bound to its high affinity receptor uPAR on the cell surface Homo sapiens 9986
-
3.4.21.73 extracellular extracellular matrix, the active form of uPA is bound to its high affinity receptor uPAR on the cell surface Homo sapiens
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.21.73 53000
-
 x * 53000, pro-uPA, SDS-PAGE Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.21.73 additional information Homo sapiens the enzyme is a serine protease involved in tissue remodeling and cell migration, the active form of uPA is bound to its high affinity receptor on the cell surface, where specific inhibitors modulate its enzymatic activity, such inhibitors also regulate the cell surface levels of uPA by triggering the internalization of the uPA-receptor-inhibitor complex via endocytosis, overview, the C-terminus of the N-terminal region contains a sequence that interacts with alphaVbeta3 integrin and is relevant for cell migration, overview ?
-
 ?
3.4.21.73 plasminogen + H2O Homo sapiens
-
 plasmin + ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.73 Homo sapiens
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.4.21.73 proteolytic modification the enzyme is produced as inactive pro-enzyme, which undergoes several post-translational modifications, the zymogen binds its own receptor uPAR and is cleaved by neighboring, membrane-bound plasmin or other proteases at K158-K159 to produce the active two-chain form held together by a single disulfide bond, further cleavage of pro-uPA at K135-K136 releases the amino-terminal fragment of uPA, overview Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.21.73 additional information the enzyme is a serine protease involved in tissue remodeling and cell migration, the active form of uPA is bound to its high affinity receptor on the cell surface, where specific inhibitors modulate its enzymatic activity, such inhibitors also regulate the cell surface levels of uPA by triggering the internalization of the uPA-receptor-inhibitor complex via endocytosis, overview, the C-terminus of the N-terminal region contains a sequence that interacts with alphaVbeta3 integrin and is relevant for cell migration, overview Homo sapiens ?
-
 ?
3.4.21.73 additional information the amino-terminal fragment ATF of uPA contains an EGF-like and a kringle domain, involved in the binding of the receptor, chain B contains the catalytic site and maintains the ability to activate plasminogen also when it is not bound to the receptor, the C-terminus of the N-terminal region contains a sequence that interacts with alphaVbeta3 integrin and is relevant for cell migration, overview Homo sapiens ?
-
 ?
3.4.21.73 plasminogen + H2O
-
 Homo sapiens plasmin + ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.21.73 ? x * 53000, pro-uPA, SDS-PAGE Homo sapiens
3.4.21.73 More the amino-terminal fragment ATF of uPA contains an EGF-like and a kringle domain, involved in the binding of the receptor, chain B contains the catalytic site and maintains the ability to activate plasminogen also when it is not bound to the receptor, the C-terminus of the N-terminal region contains a sequence that interacts with alphaVbeta3 integrin and is relevant for cell migration Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
3.4.21.73 uPA
-
 Homo sapiens
3.4.21.73 Urokinase-type plasminogen activator
-
 Homo sapiens