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Literature summary extracted from

  • Kawaguchi, M.; Yasumasu, S.; Hiroi, J.; Naruse, K.; Inoue, M.; Iuchi, I.
    Evolution of teleostean hatching enzyme genes and their paralogous genes (2006), Dev. Genes Evol., 216, 769-784.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.24.B25 cloning from teleost, three copies of MAHCE gene including one pseudogene, gene cluster, DNA and amino acid sequence determination and analysis, genetic structure, genes for hatching enzyme MAHCE are intron-less, sequence comparison and phylogenetic analysis Oryzias latipes
3.4.24.66 single copy of MLCE gene, DNA and amino acid sequence determination and analysis, genetic structure, sequence comparison and phylogenetic analysis Oryzias latipes
3.4.24.67 five copies of MHCE gene including one pseudogene, DNA and amino acid sequence determination and analysis, genetic structure, genes for hatching enzyme MHCE are intron-less, sequence comparison and phylogenetic analysis Oryzias latipes

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.24.B25 Zn2+ zinc endopeptidase Oryzias latipes
3.4.24.66 Zn2+ zinc endopeptidase Oryzias latipes
3.4.24.67 Zn2+ zinc endopeptidase Oryzias latipes

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.24.B25 chorion layer protein + H2O Oryzias latipes
-
 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.24.B25 Oryzias latipes Q14TH9 orange-red variety of medaka
-
3.4.24.66 Oryzias latipes
-
 orange-red variety of medaka
-
3.4.24.67 Oryzias latipes
-
 orange-red variety of medaka
-

Reaction

EC Number Reaction Comment Organism Reaction ID
3.4.24.B25 hydrolysis of egg envelope, solubilization of two-layered chorion the enzyme swells the hard egg envelope by its limited proteolytic action Oryzias latipes
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.24.B25 embryo the enzyme is expressed only in pre-hatching embryos, hatching liquid Oryzias latipes
-
3.4.24.66 embryo the enzyme is expressed only in pre-hatching embryos Oryzias latipes
-
3.4.24.67 embryo the enzyme is expressed only in pre-hatching embryos Oryzias latipes
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.24.B25 chorion layer protein + H2O
-
 Oryzias latipes ?
-
 ?
3.4.24.B25 chorion layer protein + H2O MAHCE is an acidic HCE, and is similar but not identical to HCE, choriolysin, EC 3.4.24.67, the enzyme swells the egg envelope by its limited proteolytic action Oryzias latipes ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.24.B25 embryo-specific hatching enzyme
-
 Oryzias latipes
3.4.24.B25 MAHCE
-
 Oryzias latipes
3.4.24.B25 medaka acidic high choriolytic enzyme
-
 Oryzias latipes
3.4.24.B25 medaka hatching enzyme
-
 Oryzias latipes
3.4.24.B25 More the enzyme is an astacin family zinc endopeptidase Oryzias latipes
3.4.24.66 choriolysin L
-
 Oryzias latipes
3.4.24.66 embryo-specific hatching enzyme
-
 Oryzias latipes
3.4.24.66 Low choriolytic enzyme
-
 Oryzias latipes
3.4.24.66 medaka hatching enzyme
-
 Oryzias latipes
3.4.24.66 medaka low choriolytic enzyme
-
 Oryzias latipes
3.4.24.66 MLCE
-
 Oryzias latipes
3.4.24.66 More the enzyme is an astacin family zinc endopeptidase Oryzias latipes
3.4.24.67 embryo-specific hatching enzyme
-
 Oryzias latipes
3.4.24.67 medaka hatching enzyme
-
 Oryzias latipes
3.4.24.67 MHCE
-
 Oryzias latipes
3.4.24.67 More the enzyme is an astacin family zinc endopeptidase Oryzias latipes