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Literature summary extracted from

  • Jin, X.; Yagi, M.; Akiyama, N.; Hirosaki, T.; Higashi, S.; Lin, C.Y.; Dickson, R.B.; Kitamura, H.; Miyazaki, K.
    Matriptase activates stromelysin (MMP-3) and promotes tumor growth and angiogenesis (2006), Cancer Sci., 97, 1327-1334.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.21.109 overexpression in stomach cancer cell line AZ521, Mat-AZ521 cells release an active form of matriptase into culture medium Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
3.4.21.109 additional information when implanting enzyme overexpressing Mat-AZ521 cells into nude mice subcutaneously or intraperitoneally, Mat-AZ521 cells grew faster and produced much larger solid tumors than the control cells, overexpression shortened the survival time of tumor-bearing mice, number and the size of blood vessels in tumor tissues are significantly higher in the Mat-AZ521 tumors than in the control cells Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.21.109 diisopropylfluorophosphate complete inhibition at 5 mM Homo sapiens
3.4.24.17 1,10-phenanthroline
-
 Mus musculus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.21.109 membrane matriptase-2 is a type II transmembrane serine protease Homo sapiens 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.21.109 Ca2+
-
 Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.21.109 75000
-
 x * 75000, native enzyme, SDS-PAGE Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.21.109 additional information Homo sapiens matriptase promotes tumor growth and angiogenesis by enhancing extracellular matrix degradation in tumor cell microenvironments, overview ?
-
 ?
3.4.21.109 stromelysin + H2O Homo sapiens activation activated stromelysin + propeptide of stromelysin
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.109 Homo sapiens
-
-
-
3.4.24.17 Mus musculus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.21.109 native enzyme from colon carcinoma cell line DLD-1 by gel filtration, dialysis, adsorption chromatography, abion exchange chromatography, and freeze-drying Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.21.109 carcinoma cell
-
 Homo sapiens
-
3.4.21.109 colon carcinoma cell
-
 Homo sapiens
-
3.4.21.109 DLD-1 cell
-
 Homo sapiens
-
3.4.21.109 epithelial cell
-
 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.21.109 additional information matriptase promotes tumor growth and angiogenesis by enhancing extracellular matrix degradation in tumor cell microenvironments, overview Homo sapiens ?
-
 ?
3.4.21.109 stromelysin + H2O activation Homo sapiens activated stromelysin + propeptide of stromelysin
-
 ?
3.4.21.109 stromelysin + H2O i.e. MMP-3 or matrix metalloproteinase-3, recombinant human substrate, activation Homo sapiens activated stromelysin + propeptide of stromelysin
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.21.109 ? x * 75000, native enzyme, SDS-PAGE Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.21.109 37
-
 assay at Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.21.109 7.5
-
 assay at Homo sapiens