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Literature summary extracted from

  • Lee, S.J.; Kim, H.S.; Kim, S.J.; Park, S.; Kim, B.J.; Shuler, M.L.; Lee, E.Y.
    Cloning, expression and enantioselective hydrolytic catalysis of a microsomal epoxide hydrolase from a marine fish, Mugil cephalus (2007), Biotechnol. Lett., 29, 237-246.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.3.2.9 synthesis enzyme prefers (R)-styrene oxide. Production of enantiopure (S)-styrene oxide by use of enzyme in batch kinetic resolution of racemic styrene oxide Mugil cephalus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.3.2.9 expression in Escherichia coli Mugil cephalus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.3.2.9 homology modeling. Structure exhibits a alpha/beta-hydrolase fold main domain with a lid domain over the active site Mugil cephalus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.3.2.9 49800
-
 x * 49800, SDS-PAGE Mugil cephalus

Organism

EC Number Organism UniProt Comment Textmining
3.3.2.9 Mugil cephalus
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.3.2.9 (R)-styrene oxide + H2O R-enantiomer is preferred Mugil cephalus (1R)-1-phenylethane-1,2-diol
-
 ?

Subunits

EC Number Subunits Comment Organism
3.3.2.9 ? x * 49800, SDS-PAGE Mugil cephalus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.3.2.9 30
-
-
 Mugil cephalus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.3.2.9 7.5
-
-
 Mugil cephalus