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Literature summary extracted from

  • Rasmussen, L.E.; Sorensen, H.R.; Vind, J.; Vikso-Nielsen, A.
    Mode of action and properties of the beta-xylosidases from Talaromyces emersonii and Trichoderma reesei (2006), Biotechnol. Bioeng., 94, 869-876.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.37 expression in Aspergillus oryzae Trichoderma reesei
3.2.1.37 expression in Aspergillus oryzae Rasamsonia emersonii

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.2.1.37 homology modeling of structure Trichoderma reesei
3.2.1.37 homology modeling of structure Rasamsonia emersonii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.37 0.06
-
 4-nitrophenyl-beta-D-xylopyranoside pH 4.0, 40°C Rasamsonia emersonii
3.2.1.37 0.08
-
 xylohexaose pH 4.0, 40°C Trichoderma reesei
3.2.1.37 0.09
-
 xylohexaose pH 4.0, 40°C Rasamsonia emersonii
3.2.1.37 0.1
-
 xylopentaose pH 4.0, 40°C Trichoderma reesei
3.2.1.37 0.13
-
 xylopentaose pH 4.0, 40°C Rasamsonia emersonii
3.2.1.37 0.15
-
 xylotetraose pH 4.0, 40°C Rasamsonia emersonii
3.2.1.37 0.16
-
 xylotetraose pH 4.0, 40°C Trichoderma reesei
3.2.1.37 0.17
-
 xylotriose pH 4.0, 40°C Rasamsonia emersonii
3.2.1.37 0.19
-
 xylobiose pH 4.0, 40°C Rasamsonia emersonii
3.2.1.37 0.2
-
 xylotriose pH 4.0, 40°C Trichoderma reesei
3.2.1.37 0.4
-
 xylobiose pH 4.0, 40°C Trichoderma reesei
3.2.1.37 0.8
-
 4-nitrophenyl-beta-D-xylopyranoside pH 4.0, 40°C Trichoderma reesei

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.37 Rasamsonia emersonii
-
-
-
3.2.1.37 Trichoderma reesei
-
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
3.2.1.37 (Xylbeta(1-4))x + H2O = beta-D-xylopyranose + (Xylbeta(1-4))x-1 active site residues are D242 and E441 Rasamsonia emersonii
a
3.2.1.37 (Xylbeta(1-4))x + H2O = beta-D-xylopyranose + (Xylbeta(1-4))x-1 active site residues are D264 and E464 Trichoderma reesei
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.37 1304
-
 pH 4.0, 60°C Trichoderma reesei
3.2.1.37 3390
-
 pH 4.0, 60°C Rasamsonia emersonii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.37 4-nitrophenyl beta-D-xylopyranoside + H2O
-
 Trichoderma reesei 4-nitrophenol + beta-D-xylose
-
 ?
3.2.1.37 4-nitrophenyl beta-D-xylopyranoside + H2O
-
 Rasamsonia emersonii 4-nitrophenol + beta-D-xylose
-
 ?
3.2.1.37 vinasse + H2O by-product of ethanol fermentation Rasamsonia emersonii D-xylose + ? 12.1% release of xylose by enzyme alone, combination of enzyme and the multicomponent enzyme product Ultraflo L results in 41.9% release of xylose ?
3.2.1.37 vinasse + H2O by-product of ethanol fermentation Trichoderma reesei D-xylose + ? 7.7% release of xylose by enzyme alone, combination of enzyme and the multicomponent enzyme product Ultraflo L results in 40.8% relaese of xylose ?
3.2.1.37 xylobiose + H2O
-
 Trichoderma reesei 2 beta-D-xylose hydrolysis via retention of anomeric configuration ?
3.2.1.37 xylobiose + H2O
-
 Rasamsonia emersonii 2 beta-D-xylose hydrolysis via retention of anomeric configuration ?
3.2.1.37 xylohexaose + H2O
-
 Trichoderma reesei beta-D-xylose
-
 ?
3.2.1.37 xylohexaose + H2O
-
 Rasamsonia emersonii beta-D-xylose
-
 ?
3.2.1.37 xylopentaose + H2O
-
 Trichoderma reesei beta-D-xylose
-
 ?
3.2.1.37 xylopentaose + H2O
-
 Rasamsonia emersonii beta-D-xylose
-
 ?
3.2.1.37 xylotetraose + H2O
-
 Trichoderma reesei xylotriose + beta-D-xylose
-
 ?
3.2.1.37 xylotetraose + H2O
-
 Rasamsonia emersonii xylotriose + beta-D-xylose
-
 ?
3.2.1.37 xylotriose + 2 H2O
-
 Trichoderma reesei 3 D-xylose
-
 ?
3.2.1.37 xylotriose + 2 H2O
-
 Rasamsonia emersonii 3 D-xylose
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.37 betaXTE
-
 Rasamsonia emersonii
3.2.1.37 betaXTR
-
 Trichoderma reesei

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.37 60
-
-
 Trichoderma reesei
3.2.1.37 60
-
-
 Rasamsonia emersonii

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.37 60
-
 stable up to Trichoderma reesei
3.2.1.37 60
-
 stable up to Rasamsonia emersonii
3.2.1.37 70
-
 30 min, complete inactivation Trichoderma reesei
3.2.1.37 80
-
 30 min, complete inactivation Rasamsonia emersonii

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.37 0.002
-
 xylobiose pH 4.0, 40°C Rasamsonia emersonii
3.2.1.37 0.002
-
 xylohexaose pH 4.0, 40°C Trichoderma reesei
3.2.1.37 0.0027
-
 xylotetraose pH 4.0, 40°C Trichoderma reesei
3.2.1.37 0.0029
-
 xylotriose pH 4.0, 40°C Trichoderma reesei
3.2.1.37 0.0029
-
 xylohexaose pH 4.0, 40°C Rasamsonia emersonii
3.2.1.37 0.003
-
 xylopentaose pH 4.0, 40°C Trichoderma reesei
3.2.1.37 0.0034
-
 xylopentaose pH 4.0, 40°C Rasamsonia emersonii
3.2.1.37 0.0036
-
 xylotetraose pH 4.0, 40°C Rasamsonia emersonii
3.2.1.37 0.0037
-
 xylotriose pH 4.0, 40°C Rasamsonia emersonii
3.2.1.37 0.0044
-
 xylobiose pH 4.0, 40°C Trichoderma reesei
3.2.1.37 0.0147
-
 4-nitrophenyl-beta-D-xylopyranoside pH 4.0, 40°C Trichoderma reesei
3.2.1.37 0.017
-
 4-nitrophenyl-beta-D-xylopyranoside pH 4.0, 40°C Rasamsonia emersonii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.37 3 3.5
-
 Trichoderma reesei
3.2.1.37 3 3.5
-
 Rasamsonia emersonii

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.37 4.5
-
 50% residual activity Rasamsonia emersonii
3.2.1.37 5.5
-
 50% residual activity Trichoderma reesei