EC Number | Cloned (Comment) | Organism |
---|---|---|
3.4.24.15 | Pz peptidases A and B overexpression in Escherichia coli strain BL21(DE3) | Geobacillus sp. |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.4.24.15 | Gly-Pro-Phe-PSI[PO2CH2]-Gly-Pro-Nle | specific for neurolysin | Geobacillus sp. | |
3.4.24.15 | Z-(D,L)-Phe-PSI[PO2CH2]-(D,L)-Ala-Lys-Ser | specific for thimet oligopeptidase | Geobacillus sp. | |
3.4.24.15 | Z-(D,L)-Phe-PSI[PO2CH2]-(D,L)-Ala-Lys-Tyr | specific for thimet oligopeptidase | Geobacillus sp. |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.4.24.15 | Collagen + H2O | Geobacillus sp. | degradation | ? | - |
? | |
3.4.24.15 | Collagen + H2O | Geobacillus sp. MO-1 | degradation | ? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.4.24.15 | Geobacillus sp. | - |
- |
- |
3.4.24.15 | Geobacillus sp. MO-1 | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.4.24.15 | recombinant Pz peptidases A and B from Escherichia coli strain BL21(DE3) by heat denaturation, anion exchange chromatography and gel filtration, 2.2fold and 8.4fold, respectively | Geobacillus sp. |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.4.24.15 | 19.5 | - |
purified recombinant Pz peptidase B | Geobacillus sp. |
3.4.24.15 | 30.4 | - |
purified recombinant Pz peptidase A | Geobacillus sp. |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.4.24.15 | (7-methoxycoumarin-4-yl)-acetyl-Pro-Leu-Gly-Pro-D-Lys(2,4-dinitrophenyl) + H2O | - |
Geobacillus sp. | ? | - |
? | |
3.4.24.15 | (7-methoxycoumarin-4-yl)-acetyl-Pro-Leu-Gly-Pro-D-Lys(2,4-dinitrophenyl) + H2O | - |
Geobacillus sp. MO-1 | ? | - |
? | |
3.4.24.15 | Collagen + H2O | degradation | Geobacillus sp. | ? | - |
? | |
3.4.24.15 | Collagen + H2O | the enzyme recognizes the specific sequence Gly-Pro-Xaa of in collagen | Geobacillus sp. | ? | - |
? | |
3.4.24.15 | Collagen + H2O | degradation | Geobacillus sp. MO-1 | ? | - |
? | |
3.4.24.15 | Collagen + H2O | the enzyme recognizes the specific sequence Gly-Pro-Xaa of in collagen | Geobacillus sp. MO-1 | ? | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.4.24.15 | endopeptidase 24-15 | - |
Geobacillus sp. |
3.4.24.15 | Pz peptidase A | - |
Geobacillus sp. |
3.4.24.15 | Pz peptidase B | - |
Geobacillus sp. |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.4.24.15 | 0.0000192 | - |
Gly-Pro-Phe-PSI[PO2CH2]-Gly-Pro-Nle | recombinant Pz peptidase B | Geobacillus sp. | |
3.4.24.15 | 0.0000378 | - |
Z-(D,L)-Phe-PSI[PO2CH2]-(D,L)-Ala-Lys-Ser | recombinant Pz peptidase B | Geobacillus sp. | |
3.4.24.15 | 0.000043 | - |
Z-(D,L)-Phe-PSI[PO2CH2]-(D,L)-Ala-Lys-Tyr | recombinant Pz peptidase B | Geobacillus sp. | |
3.4.24.15 | 0.0000877 | - |
Z-(D,L)-Phe-PSI[PO2CH2]-(D,L)-Ala-Lys-Tyr | recombinant Pz peptidase A | Geobacillus sp. | |
3.4.24.15 | 0.0000889 | - |
Gly-Pro-Phe-PSI[PO2CH2]-Gly-Pro-Nle | recombinant Pz peptidase A | Geobacillus sp. | |
3.4.24.15 | 0.0000901 | - |
Z-(D,L)-Phe-PSI[PO2CH2]-(D,L)-Ala-Lys-Ser | recombinant Pz peptidase A | Geobacillus sp. |