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Literature summary extracted from

  • Sugihara, Y.; Kawasaki, A.; Tsujimoto, Y.; Matsui, H.; Watanabe, K.
    Potencies of phosphine peptide inhibitors of mammalian thimet oligopeptidase and neurolysin on two bacterial pz peptidases (2007), Biosci. Biotechnol. Biochem., 71, 594-597.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.24.15 Pz peptidases A and B overexpression in Escherichia coli strain BL21(DE3) Geobacillus sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.24.15 Gly-Pro-Phe-PSI[PO2CH2]-Gly-Pro-Nle specific for neurolysin Geobacillus sp.
3.4.24.15 Z-(D,L)-Phe-PSI[PO2CH2]-(D,L)-Ala-Lys-Ser specific for thimet oligopeptidase Geobacillus sp.
3.4.24.15 Z-(D,L)-Phe-PSI[PO2CH2]-(D,L)-Ala-Lys-Tyr specific for thimet oligopeptidase Geobacillus sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.24.15 Collagen + H2O Geobacillus sp. degradation ?
-
?
3.4.24.15 Collagen + H2O Geobacillus sp. MO-1 degradation ?
-
?

Organism

EC Number Organism UniProt Comment Textmining
3.4.24.15 Geobacillus sp.
-
-
-
3.4.24.15 Geobacillus sp. MO-1
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.24.15 recombinant Pz peptidases A and B from Escherichia coli strain BL21(DE3) by heat denaturation, anion exchange chromatography and gel filtration, 2.2fold and 8.4fold, respectively Geobacillus sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.24.15 19.5
-
purified recombinant Pz peptidase B Geobacillus sp.
3.4.24.15 30.4
-
purified recombinant Pz peptidase A Geobacillus sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.24.15 (7-methoxycoumarin-4-yl)-acetyl-Pro-Leu-Gly-Pro-D-Lys(2,4-dinitrophenyl) + H2O
-
Geobacillus sp. ?
-
?
3.4.24.15 (7-methoxycoumarin-4-yl)-acetyl-Pro-Leu-Gly-Pro-D-Lys(2,4-dinitrophenyl) + H2O
-
Geobacillus sp. MO-1 ?
-
?
3.4.24.15 Collagen + H2O degradation Geobacillus sp. ?
-
?
3.4.24.15 Collagen + H2O the enzyme recognizes the specific sequence Gly-Pro-Xaa of in collagen Geobacillus sp. ?
-
?
3.4.24.15 Collagen + H2O degradation Geobacillus sp. MO-1 ?
-
?
3.4.24.15 Collagen + H2O the enzyme recognizes the specific sequence Gly-Pro-Xaa of in collagen Geobacillus sp. MO-1 ?
-
?

Synonyms

EC Number Synonyms Comment Organism
3.4.24.15 endopeptidase 24-15
-
Geobacillus sp.
3.4.24.15 Pz peptidase A
-
Geobacillus sp.
3.4.24.15 Pz peptidase B
-
Geobacillus sp.

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.4.24.15 0.0000192
-
Gly-Pro-Phe-PSI[PO2CH2]-Gly-Pro-Nle recombinant Pz peptidase B Geobacillus sp.
3.4.24.15 0.0000378
-
Z-(D,L)-Phe-PSI[PO2CH2]-(D,L)-Ala-Lys-Ser recombinant Pz peptidase B Geobacillus sp.
3.4.24.15 0.000043
-
Z-(D,L)-Phe-PSI[PO2CH2]-(D,L)-Ala-Lys-Tyr recombinant Pz peptidase B Geobacillus sp.
3.4.24.15 0.0000877
-
Z-(D,L)-Phe-PSI[PO2CH2]-(D,L)-Ala-Lys-Tyr recombinant Pz peptidase A Geobacillus sp.
3.4.24.15 0.0000889
-
Gly-Pro-Phe-PSI[PO2CH2]-Gly-Pro-Nle recombinant Pz peptidase A Geobacillus sp.
3.4.24.15 0.0000901
-
Z-(D,L)-Phe-PSI[PO2CH2]-(D,L)-Ala-Lys-Ser recombinant Pz peptidase A Geobacillus sp.