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Literature summary extracted from

  • Shi, D.; Yu, X.; Roth, L.; Tuchman, M.; Allewell, N.M.
    Structure of a novel N-acetyl-L-citrulline deacetylase from Xanthomonas campestris (2007), Biophys. Chem., 126, 86-93.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.1.B4 expressed as His-tag fusion protein in Escherichia coli BL21(DE3) Xanthomonas campestris

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.5.1.B4 His-tag free protein, hanging drop vapor diffusion method Xanthomonas campestris

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.1.B4 Co2+ bound to active site, coordinated by His72, Asp103, Glu155, and one water molecule Xanthomonas campestris

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.1.B4 N2-acetyl-L-citrulline + H2O Xanthomonas campestris involved in an alternative pathway for arginine synthesis L-citrulline + acetate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.B4 Xanthomonas campestris
-
 strain ATCC 33913D
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.B4 recombinant protein using His-tag Xanthomonas campestris

Reaction

EC Number Reaction Comment Organism Reaction ID
3.5.1.B4 N-acetyl-L-citrulline + H2O = L-citrulline + acetate Glu130 functions as a general acid–base catalyst, activating a water molecule, while the Co2+ ion interacts with the acetyl group to polarize the amide bond Xanthomonas campestris
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.B4 N2-acetyl-L-citrulline + H2O
-
 Xanthomonas campestris L-citrulline + acetate
-
 ?
3.5.1.B4 N2-acetyl-L-citrulline + H2O involved in an alternative pathway for arginine synthesis Xanthomonas campestris L-citrulline + acetate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.1.B4 dimer crystal structure analysis Xanthomonas campestris

Synonyms

EC Number Synonyms Comment Organism
3.5.1.B4 aCDase
-
 Xanthomonas campestris
3.5.1.B4 argE' gene product
-
 Xanthomonas campestris