BRENDA - Enzyme Database

Human dipeptidyl peptidase III acts as a post-proline-cleaving enzyme on endomorphins

Barsun, M.; Jajcanin, N.; Vukeli?, B.; Spoljari?, J.; Abrami?, M.; Biol. Chem. 388, 343-348 (2007) View publication on PubMed

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.4.14.4
Gly-Gly-Phe-Leu
enkephalin fragment
Homo sapiens
3.4.14.4
Leu-Val-Val-Tyr-Pro-Trp-Thr-Gln-Arg
Leu-valorphin-Arg
Homo sapiens
3.4.14.4
Tyr(SO3H)-Gly-Gly-Phe-Leu
-
Homo sapiens
3.4.14.4
Tyr-Ala-Gly-Phe-Leu
Ala2-Leu-enkephalin
Homo sapiens
3.4.14.4
Tyr-D-Ala-Gly-Phe-Leu
D-Ala2-Leu-enkephalin
Homo sapiens
3.4.14.4
Tyr-Gly-Gly-Phe
enkephalin fragment
Homo sapiens
3.4.14.4
Tyr-Gly-Gly-Phe-Leu
Leu-enkephalin
Homo sapiens
3.4.14.4
Tyr-Gly-Gly-Phe-Leu-Arg-Arg-Ile
dynorphin A 1-8
Homo sapiens
3.4.14.4
Tyr-Pro-Phe-Phe-NH2
endomorphin-2
Homo sapiens
3.4.14.4
Tyr-Pro-Phe-Val-Glu-Pro-Ile
human beta-casomorphin
Homo sapiens
3.4.14.4
Tyr-Pro-Trp-Phe-NH2
endomorphin-1
Homo sapiens
3.4.14.4
Val-Val-Tyr-Pro-Trp-Thr-Gln
valorphin
Homo sapiens
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.4.14.4
0.0081
-
Tyr-Pro-Trp-Phe-NH2
endomorphin-1
Homo sapiens
3.4.14.4
0.0222
-
Arg-Arg-2-naphthylamide
-
Homo sapiens
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
3.4.14.4
Zn2+
-
Homo sapiens
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.4.14.4
82000
-
determined by PAGE, native and denaturing conditions
Homo sapiens
Organism
EC Number
Organism
UniProt
Commentary
Textmining
3.4.14.4
Homo sapiens
-
-
-
Purification (Commentary)
EC Number
Purification (Commentary)
Organism
3.4.14.4
the protein is purified from human erythrocytes
Homo sapiens
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
3.4.14.4
cerebrospinal fluid
-
Homo sapiens
-
3.4.14.4
erythrocyte
-
Homo sapiens
-
3.4.14.4
spinal cord
superficial laminae of the spinal cord dorsal horn
Homo sapiens
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.4.14.4
87.5
-
in the presence of 10 microM CoCl2
Homo sapiens
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
3.4.14.4
Arg-Arg-2-naphthylamide + H2O
substrate used in the activity assay
683203
Homo sapiens
Arg-Arg + 2-naphthylamine
-
-
-
?
3.4.14.4
Tyr-Pro-Trp-Phe-NH2 + H2O
endomorphin-1
683203
Homo sapiens
Tyr-Pro + Trp-Phe-NH2
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
3.4.14.4
monomer
-
Homo sapiens
Synonyms
EC Number
Synonyms
Commentary
Organism
3.4.14.4
dipeptidyl arylamidase III
-
Homo sapiens
3.4.14.4
dipeptidyl peptidase III
-
Homo sapiens
3.4.14.4
DPP III
-
Homo sapiens
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
3.4.14.4
37
-
-
Homo sapiens
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
3.4.14.4
2
8
Arg-Arg-2-naphthylamide
-
Homo sapiens
3.4.14.4
5
-
Tyr-Pro-Trp-Phe-NH2
endomorphin-1
Homo sapiens
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.4.14.4
7.4
-
physiological condition, determination of Ki values and kinetic parameters of the hydrolysis of endomorphin-1
Homo sapiens
3.4.14.4
8.6
-
assay condition with the synthetic substrate Arg-Arg-2-naphthylamide, determination of specific activity
Homo sapiens
Ki Value [mM]
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
3.4.14.4
0.000049
-
Val-Val-Tyr-Pro-Trp-Thr-Gln
-
Homo sapiens
3.4.14.4
0.000375
-
Tyr-Ala-Gly-Phe-Leu
-
Homo sapiens
3.4.14.4
0.00056
-
Tyr-Pro-Phe-Val-Glu-Pro-Ile
-
Homo sapiens
3.4.14.4
0.00249
-
Tyr-Pro-Phe-Phe-NH2
-
Homo sapiens
3.4.14.4
0.00341
-
Leu-Val-Val-Tyr-Pro-Trp-Thr-Gln-Arg
-
Homo sapiens
3.4.14.4
0.00365
-
Tyr-Gly-Gly-Phe-Leu
-
Homo sapiens
3.4.14.4
0.004
-
Tyr-Gly-Gly-Phe
-
Homo sapiens
3.4.14.4
0.005
-
Tyr-Pro-Trp-Phe-NH2
-
Homo sapiens
3.4.14.4
0.0235
-
Gly-Gly-Phe-Leu
-
Homo sapiens
3.4.14.4
0.027
-
Tyr-D-Ala-Gly-Phe-Leu
-
Homo sapiens
3.4.14.4
0.0434
-
Tyr(SO3H)-Gly-Gly-Phe-Leu
-
Homo sapiens
3.4.14.4
0.0699
-
Tyr-Gly-Gly-Phe-Leu-Arg-Arg-Ile
-
Homo sapiens
pI Value
EC Number
Organism
Commentary
pI Value Maximum
pI Value
3.4.14.4
Homo sapiens
-
-
4.5
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.4.14.4
Gly-Gly-Phe-Leu
enkephalin fragment
Homo sapiens
3.4.14.4
Leu-Val-Val-Tyr-Pro-Trp-Thr-Gln-Arg
Leu-valorphin-Arg
Homo sapiens
3.4.14.4
Tyr(SO3H)-Gly-Gly-Phe-Leu
-
Homo sapiens
3.4.14.4
Tyr-Ala-Gly-Phe-Leu
Ala2-Leu-enkephalin
Homo sapiens
3.4.14.4
Tyr-D-Ala-Gly-Phe-Leu
D-Ala2-Leu-enkephalin
Homo sapiens
3.4.14.4
Tyr-Gly-Gly-Phe
enkephalin fragment
Homo sapiens
3.4.14.4
Tyr-Gly-Gly-Phe-Leu
Leu-enkephalin
Homo sapiens
3.4.14.4
Tyr-Gly-Gly-Phe-Leu-Arg-Arg-Ile
dynorphin A 1-8
Homo sapiens
3.4.14.4
Tyr-Pro-Phe-Phe-NH2
endomorphin-2
Homo sapiens
3.4.14.4
Tyr-Pro-Phe-Val-Glu-Pro-Ile
human beta-casomorphin
Homo sapiens
3.4.14.4
Tyr-Pro-Trp-Phe-NH2
endomorphin-1
Homo sapiens
3.4.14.4
Val-Val-Tyr-Pro-Trp-Thr-Gln
valorphin
Homo sapiens
Ki Value [mM] (protein specific)
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
3.4.14.4
0.000049
-
Val-Val-Tyr-Pro-Trp-Thr-Gln
-
Homo sapiens
3.4.14.4
0.000375
-
Tyr-Ala-Gly-Phe-Leu
-
Homo sapiens
3.4.14.4
0.00056
-
Tyr-Pro-Phe-Val-Glu-Pro-Ile
-
Homo sapiens
3.4.14.4
0.00249
-
Tyr-Pro-Phe-Phe-NH2
-
Homo sapiens
3.4.14.4
0.00341
-
Leu-Val-Val-Tyr-Pro-Trp-Thr-Gln-Arg
-
Homo sapiens
3.4.14.4
0.00365
-
Tyr-Gly-Gly-Phe-Leu
-
Homo sapiens
3.4.14.4
0.004
-
Tyr-Gly-Gly-Phe
-
Homo sapiens
3.4.14.4
0.005
-
Tyr-Pro-Trp-Phe-NH2
-
Homo sapiens
3.4.14.4
0.0235
-
Gly-Gly-Phe-Leu
-
Homo sapiens
3.4.14.4
0.027
-
Tyr-D-Ala-Gly-Phe-Leu
-
Homo sapiens
3.4.14.4
0.0434
-
Tyr(SO3H)-Gly-Gly-Phe-Leu
-
Homo sapiens
3.4.14.4
0.0699
-
Tyr-Gly-Gly-Phe-Leu-Arg-Arg-Ile
-
Homo sapiens
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.4.14.4
0.0081
-
Tyr-Pro-Trp-Phe-NH2
endomorphin-1
Homo sapiens
3.4.14.4
0.0222
-
Arg-Arg-2-naphthylamide
-
Homo sapiens
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
3.4.14.4
Zn2+
-
Homo sapiens
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.4.14.4
82000
-
determined by PAGE, native and denaturing conditions
Homo sapiens
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.4.14.4
the protein is purified from human erythrocytes
Homo sapiens
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
3.4.14.4
cerebrospinal fluid
-
Homo sapiens
-
3.4.14.4
erythrocyte
-
Homo sapiens
-
3.4.14.4
spinal cord
superficial laminae of the spinal cord dorsal horn
Homo sapiens
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.4.14.4
87.5
-
in the presence of 10 microM CoCl2
Homo sapiens
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
3.4.14.4
Arg-Arg-2-naphthylamide + H2O
substrate used in the activity assay
683203
Homo sapiens
Arg-Arg + 2-naphthylamine
-
-
-
?
3.4.14.4
Tyr-Pro-Trp-Phe-NH2 + H2O
endomorphin-1
683203
Homo sapiens
Tyr-Pro + Trp-Phe-NH2
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
3.4.14.4
monomer
-
Homo sapiens
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
3.4.14.4
37
-
-
Homo sapiens
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
3.4.14.4
2
8
Arg-Arg-2-naphthylamide
-
Homo sapiens
3.4.14.4
5
-
Tyr-Pro-Trp-Phe-NH2
endomorphin-1
Homo sapiens
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.4.14.4
7.4
-
physiological condition, determination of Ki values and kinetic parameters of the hydrolysis of endomorphin-1
Homo sapiens
3.4.14.4
8.6
-
assay condition with the synthetic substrate Arg-Arg-2-naphthylamide, determination of specific activity
Homo sapiens
pI Value (protein specific)
EC Number
Organism
Commentary
pI Value Maximum
pI Value
3.4.14.4
Homo sapiens
-
-
4.5