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Literature summary extracted from
- Neutrophil elastase, proteinase 3 and cathepsin G: physicochemical properties, activity and physiopathological functions (2008), Biochimie, 90, 227-242.
Application
| EC Number | Application | Comment | Organism |
|---|---|---|---|
| 3.4.21.20 | drug development | the enzyme is a therapeutic target | Homo sapiens |
| 3.4.21.76 | medicine | responsible for eliminating intracellular pathogens and breaking down tissues at inflammatory sites. Possible molecular target for anti-inflammatory agents. Involved in degradation of ECM components and cleavage of inflammatory mediators, platlet activation, induction of endothelial cells and apoptosis, negative feedback regulation of granulopoiesis. Shows bactericidal properties. Is a target of auto-antibodies in Wegeners granulomatosis | Homo sapiens |
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.4.21.20 | the gene is located at chromosome 14q11.2 | Homo sapiens |
| 3.4.21.76 | expressed in insect cells | Homo sapiens |
Crystallization (Commentary)
| EC Number | Crystallization (Comment) | Organism |
|---|---|---|
| 3.4.21.76 | crystal structure of recombinant PR3. Overall fold consists of two beta-barrel domains. PR3 structure includes a disaccharide unit covalently attached to Asn159. PR3 substrate binding sites at S4 to S3' | Homo sapiens |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.4.21.20 | alpha1-Aantichymotrypsin | - |
Homo sapiens | |
| 3.4.21.20 | alpha1-protease inhibitor | - |
Homo sapiens | |
| 3.4.21.20 | alpha2-Macroglobulin | - |
Homo sapiens | |
| 3.4.21.20 | chelonianin | - |
Homo sapiens | |
| 3.4.21.20 | monocyte neutrophil elastase inhibitor | - |
Homo sapiens | |
| 3.4.21.20 | additional information | inhibition mechanisms, overview | Homo sapiens | |
| 3.4.21.20 | protease inhibitor PI6 | - |
Homo sapiens | |
| 3.4.21.20 | secretory leukocyteprotease inhibitor | - |
Homo sapiens | |
| 3.4.21.20 | serpin | - |
Homo sapiens | |
| 3.4.21.20 | squamous cell carcinoma antigen 2 | - |
Homo sapiens | |
| 3.4.21.76 | alpha1-PI | - |
Homo sapiens | |
| 3.4.21.76 | alpha2-Macroglobulin | - |
Homo sapiens | |
| 3.4.21.76 | elafin | - |
Homo sapiens | |
| 3.4.21.76 | MNEI | - |
Homo sapiens | |
| 3.4.21.76 | additional information | transcription of PR3 is downregulated upon granulocyte and monocyte maturation | Homo sapiens | |
| 3.4.21.76 | pre-elafin | - |
Homo sapiens |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.4.21.20 | cell surface | active enzyme | Homo sapiens | 9986 | - |
| 3.4.21.20 | extracellular | active enzyme | Homo sapiens | - |
- |
| 3.4.21.76 | cell surface | granulocytes and monocytes | Homo sapiens | 9986 | - |
| 3.4.21.76 | membrane | secretory vesicles and secondary granules | Homo sapiens | 16020 | - |
| 3.4.21.76 | secondary granule | - |
Homo sapiens | - |
- |
| 3.4.21.76 | secretory vesicle | - |
Homo sapiens | 99503 | - |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.4.21.20 | 28500 | - |
x * 28500 | Homo sapiens |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.4.21.20 | additional information | Homo sapiens | the enzyme is one of the major components of the neutrophil primary granules that participate in the non-oxidative pathway of intracellular pathogen destruction, it helps kill bacterial cells and is involved in the degradation of extracellular matrix components during acute and chronic inflammation, the enzyme is important in regulation of immune response, control of cellular signaling through the procession of cytokines and modulation of the cytokine network, biological functions and roles in diseases of cathepsin G, overview | ? | - |
? |  |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.4.21.20 | Homo sapiens | P08311 | - |
- |
| 3.4.21.76 | Homo sapiens | P24158 | - |
- |
Posttranslational Modification
| EC Number | Posttranslational Modification | Comment | Organism |
|---|---|---|---|
| 3.4.21.20 | glycoprotein | the enzyme contains one glycosylation site | Homo sapiens |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.4.21.20 | monocyte | enzyme synthesis | Homo sapiens | - |
| 3.4.21.20 | neutrophil | polymorphonuclear cells, enzyme synthesis | Homo sapiens | - |
| 3.4.21.76 | basophil | - |
Homo sapiens | - |
| 3.4.21.76 | epithelial cell | inflamed oral epithelial cell | Homo sapiens | - |
| 3.4.21.76 | granulocyte | active PR3 stored in neutrophil azurophil granules | Homo sapiens | - |
| 3.4.21.76 | monocyte | - |
Homo sapiens | - |
| 3.4.21.76 | additional information | mRNA for PR3 is transcribed in the promyelocyte and promonocyte stages of myeloid differentiation. Present in mastocytes and no-hematopoietic cell | Homo sapiens | - |
| 3.4.21.76 | neutrophil | blood neutrophils from patients with active Wegeners granulomatosis bear greater amounts of PR3 on their cell membranes than do neutrophils from healthy individuals | Homo sapiens | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.4.21.20 | Abz-EPFWEDQ-EDDnp + H2O | - |
Homo sapiens | ? | - |
? | |
| 3.4.21.20 | additional information | the enzyme is one of the major components of the neutrophil primary granules that participate in the non-oxidative pathway of intracellular pathogen destruction, it helps kill bacterial cells and is involved in the degradation of extracellular matrix components during acute and chronic inflammation, the enzyme is important in regulation of immune response, control of cellular signaling through the procession of cytokines and modulation of the cytokine network, biological functions and roles in diseases of cathepsin G, overview | Homo sapiens | ? | - |
? | |
| 3.4.21.20 | additional information | cathepsin G prefers aromatic or positively charged residues at P1 position, e.g. Phe, Tyr, Lys, or Arg, substrate specificity and active site structure analysis, overview | Homo sapiens | ? | - |
? | |
| 3.4.21.76 | Abz-VADCRDRQ-EDDnp + H2O | best synthetic substrate | Homo sapiens | Abz-VADCR + DRQ-EDDnp | - |
? | |
| 3.4.21.76 | Elastin + H2O | - |
Homo sapiens | Hydrolyzed elastin | - |
? | |
| 3.4.21.76 | FRET + H2O | - |
Homo sapiens | ? | - |
? | |
| 3.4.21.76 | IL-18 + H2O | - |
Homo sapiens | ? | - |
? | |
| 3.4.21.76 | IL-1beta + H2O | - |
Homo sapiens | ? | - |
? | |
| 3.4.21.76 | additional information | substrate specificity for small hydrophobic residues at P1 position (Val, Cys, Ala, Met, Ser and Leu) | Homo sapiens | ? | - |
? | |
| 3.4.21.76 | NF-kappaB + H2O | - |
Homo sapiens | ? | - |
? | |
| 3.4.21.76 | oxidized insulin B chain + H2O | - |
Homo sapiens | ? | - |
? | |
| 3.4.21.76 | p21 + H2O | - |
Homo sapiens | ? | - |
? | |
| 3.4.21.76 | protease-activated receptor-2 + H2O | - |
Homo sapiens | ? | - |
? | |
| 3.4.21.76 | TNF-alpha + H2O | - |
Homo sapiens | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.4.21.20 | ? | x * 28500 | Homo sapiens |
| 3.4.21.76 | ? | 29000-32000, SDS-PAGE | Homo sapiens |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.4.21.20 | More | the enzyme belongs to the chymotrypsin family | Homo sapiens |
| 3.4.21.76 | PR3 | - |
Homo sapiens |
| 3.4.21.76 | proteinase 3 | - |
Homo sapiens |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.4.21.20 | 7.5 | - |
- |
Homo sapiens |
| 3.4.21.76 | 8 | - |
- |
Homo sapiens |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.4.21.76 | additional information | - |
the mature PR3 has an inactive conformation in the acidic environment of the azurophilic granules, and becomes active upon translocation to a neutral pH environment | Homo sapiens |
pI Value
| EC Number | Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|---|
| 3.4.21.20 | Homo sapiens | about | - |
12 |
| 3.4.21.76 | Homo sapiens | - |
- |
9.5 |
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