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Literature summary extracted from

  • Deiteren, K.; Surpateanu, G.; Gilany, K.; Willemse, J.L.; Hendriks, D.F.; Augustyns, K.; Laroche, Y.; Scharpe, S.; Lambeir, A.M.
    The role of the S1 binding site of carboxypeptidase M in substrate specificity and turn-over (2007), Biochim. Biophys. Acta, 1774, 267-277.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.17.12 placental cDNA is incorporated in the pICZalphaA shuttle vector, signal peptide and C-terminal hydrophobic membrane anchor signal are removed Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.17.12 MERGETPA
-
 Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.17.12 0.027
-
 Leu-Ala-Arg CPMwt, pH 7.4, 37°C Homo sapiens
3.4.17.12 0.0277
-
 bradykinin(-Phe-Arg) CPMwt, pH 7.2, 37°C Homo sapiens
3.4.17.12 0.028
-
 His-Leu-Gly-Leu-Ala-Arg CPMwt, pH 7.4, 37°C Homo sapiens
3.4.17.12 0.033
-
 Ala-Ser-His-Leu-Gly-Leu-Ala-Arg CPMwt, pH 7.4, 37°C Homo sapiens
3.4.17.12 0.035
-
 Pro-Gly-Lys-Ala-Arg CPMwt, pH 7.2, 37°C Homo sapiens
3.4.17.12 0.041
-
 Leu-Gly-Leu-Ala-Arg CPMwt, pH 7.4, 37°C Homo sapiens
3.4.17.12 0.046
-
 benzoyl-Gly-argininic acid CPMwt, pH 7.2, 37°C Homo sapiens
3.4.17.12 0.048
-
 benzoyl-Tyr-Arg CPMwt, pH 7.4, 37°C Homo sapiens
3.4.17.12 0.049
-
 benzoyl-Gly-argininic acid hCPM, pH 7.2, 37°C Homo sapiens
3.4.17.12 0.05
-
 Gly-Leu-Ala-Arg CPMwt, pH 7.4, 37°C Homo sapiens
3.4.17.12 0.051
-
 Ser-His-Leu-Gly-Leu-Ala-Arg CPMwt, pH 7.4, 37°C Homo sapiens
3.4.17.12 0.0584
-
 benzoyl-Phe-Arg CPMwt, pH 7.4, 37°C Homo sapiens
3.4.17.12 0.068
-
 furylacryloyl-Ala-Arg hCPM, pH 7.2, 37°C Homo sapiens
3.4.17.12 0.069
-
 benzoyl-Trp-Arg CPMwt, pH 7.4, 37°C Homo sapiens
3.4.17.12 0.073
-
 Dansyl-Ala-Arg hCPM, pH 7.2, 37°C Homo sapiens
3.4.17.12 0.083
-
 Dansyl-Ala-Arg CPMwt, pH 7.2, 37°C Homo sapiens
3.4.17.12 0.09
-
 furylacryloyl-Ala-Arg CPMwt, pH 7.2, 37°C Homo sapiens
3.4.17.12 0.115
-
 benzoyl-Ala-Arg CPMwt, pH 7.4, 37°C Homo sapiens
3.4.17.12 0.127
-
 benzoyl-Gln-Arg CPMwt, pH 7.4, 37°C Homo sapiens
3.4.17.12 0.13
-
 benzoyl-Met-Arg CPMwt, pH 7.2, 37°C Homo sapiens
3.4.17.12 0.137
-
 benzoyl-Met-Arg hCPM, pH 7.2, 37°C Homo sapiens
3.4.17.12 0.138
-
 benzoyl-Met-Arg CPMwt, pH 7.4, 37°C Homo sapiens
3.4.17.12 0.162
-
 benzoyl-Ala-Arg CPMwt, pH 7.2, 37°C Homo sapiens
3.4.17.12 0.168
-
 benzoyl-Leu-Arg CPMwt, pH 7.4, 37°C Homo sapiens
3.4.17.12 0.18
-
 benzoyl-Ala-Arg hCPM, pH 7.2, 37°C Homo sapiens
3.4.17.12 0.192
-
 benzoyl-Thr-Arg CPMwt, pH 7.4, 37°C Homo sapiens
3.4.17.12 0.205
-
 benzoyl-Ile-Arg CPMwt, pH 7.4, 37°C Homo sapiens
3.4.17.12 0.206
-
 N-(4-hydroxybenzoyl)-Gly-Arg CPMwt, pH 7.2, 37°C Homo sapiens
3.4.17.12 0.219
-
 benzoyl-Ser-Arg CPMwt, pH 7.4, 37°C Homo sapiens
3.4.17.12 0.239
-
 benzoyl-His-Arg CPMwt, pH 7.4, 37°C Homo sapiens
3.4.17.12 0.241
-
 N-(4-hydroxybenzoyl)-Gly-Arg hCPM, pH 7.2, 37°C Homo sapiens
3.4.17.12 0.255
-
 benzoyl-Gly-Arg CPMwt, pH 7.2, 37°C Homo sapiens
3.4.17.12 0.264
-
 benzoyl-Val-Arg CPMwt, pH 7.4, 37°C Homo sapiens
3.4.17.12 0.289
-
 benzoyl-Gly-Arg CPMwt, pH 7.4, 37°C Homo sapiens
3.4.17.12 0.29
-
 benzoyl-Gly-Arg hCPM, pH 7.2, 37°C Homo sapiens
3.4.17.12 0.343
-
 benzoyl-Asn-Arg CPMwt, pH 7.4, 37°C Homo sapiens
3.4.17.12 1.152
-
 furylacryloyl-Ala-Lys CPMwt, pH 7.2, 37°C Homo sapiens
3.4.17.12 1.181
-
 Gly-Gly-Arg CPMwt, pH 7.2, 37°C Homo sapiens
3.4.17.12 10.15
-
 Benzoyl-Gly-Lys hCPM, pH 7.2, 37°C Homo sapiens
3.4.17.12 10.65
-
 Benzoyl-Gly-Lys CPMwt, pH 7.2, 37°C Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.17.12 membrane
-
 Homo sapiens 16020
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.17.12 49000
-
 determined by SDS-PAGE Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
3.4.17.12 Homo sapiens P14384
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.4.17.12 additional information hCPM and CPMwt differ in the nature and relative amount of the N-glycan carbohydrate moities Homo sapiens

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.17.12 wild type carboxypeptidase M, CPMwt, is purified from Pichia pastoris supernatant using hydrophobic interaction chromatography and affinity chromatography, hCPM is purified from human prostasomes Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.17.12 placenta
-
 Homo sapiens
-
3.4.17.12 prostasome
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.17.12 14
-
 CPMwt with dansyl-Ala-Arg Homo sapiens
3.4.17.12 19
-
 hCPM with dansyl-Ala-Arg Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.17.12 Ala-Ser-His-Leu-Gly-Leu-Ala-Arg + H2O human anaphylatoxin C3A fragment 70-77 Homo sapiens Ala-Ser-His-Leu-Gly-Leu-Ala + Arg
-
 ?
3.4.17.12 benzoyl-Ala-Arg + H2O
-
 Homo sapiens benzoyl-Ala + Arg
-
 ?
3.4.17.12 benzoyl-Asn-Arg + H2O
-
 Homo sapiens benzoyl-Asn + Arg
-
 ?
3.4.17.12 benzoyl-Gln-Arg + H2O
-
 Homo sapiens benzoyl-Gln + Arg
-
 ?
3.4.17.12 benzoyl-Gly-Arg + H2O
-
 Homo sapiens benzoyl-Gly + Arg
-
 ?
3.4.17.12 benzoyl-Gly-argininic acid + H2O
-
 Homo sapiens ?
-
 ?
3.4.17.12 benzoyl-Gly-Lys + H2O
-
 Homo sapiens benzoyl-Gly + Lys
-
 ?
3.4.17.12 benzoyl-His-Arg + H2O
-
 Homo sapiens benzoyl-His + Arg
-
 ?
3.4.17.12 benzoyl-Ile-Arg + H2O
-
 Homo sapiens benzoyl-Ile + Arg
-
 ?
3.4.17.12 benzoyl-Leu-Arg + H2O
-
 Homo sapiens benzoyl-Leu + Arg
-
 ?
3.4.17.12 benzoyl-Met-Arg + H2O
-
 Homo sapiens benzoyl-Met + Arg
-
 ?
3.4.17.12 benzoyl-Phe-Arg + H2O
-
 Homo sapiens benzoyl-Phe + Arg
-
 ?
3.4.17.12 benzoyl-Ser-Arg + H2O
-
 Homo sapiens benzoyl-Ser + Arg
-
 ?
3.4.17.12 benzoyl-Thr-Arg + H2O
-
 Homo sapiens benzoyl-Thr + Arg
-
 ?
3.4.17.12 benzoyl-Trp-Arg + H2O
-
 Homo sapiens benzoyl-Trp + Arg
-
 ?
3.4.17.12 benzoyl-Tyr-Arg + H2O
-
 Homo sapiens benzoyl-Tyr + Arg
-
 ?
3.4.17.12 benzoyl-Val-Arg + H2O
-
 Homo sapiens benzoyl-Val + Arg
-
 ?
3.4.17.12 bradykinin(-Phe-Arg) + H2O
-
 Homo sapiens ?
-
 ?
3.4.17.12 Dansyl-Ala-Arg + H2O enzyme assay Homo sapiens Dansyl-Ala + Arg
-
 ?
3.4.17.12 furylacryloyl-Ala-Arg + H2O
-
 Homo sapiens furylacryloyl-Ala + Arg
-
 ?
3.4.17.12 furylacryloyl-Ala-Lys + H2O
-
 Homo sapiens furylacryloyl-Ala + Lys
-
 ?
3.4.17.12 Gly-Gly-Arg + H2O
-
 Homo sapiens Gly-Gly + Arg
-
 ?
3.4.17.12 Gly-Leu-Ala-Arg + H2O human anaphylatoxin C3A fragment 74-77 Homo sapiens Gly-Leu-Ala + Arg
-
 ?
3.4.17.12 His-Leu-Gly-Leu-Ala-Arg + H2O human anaphylatoxin C3A fragment 72-77 Homo sapiens His-Leu-Gly-Leu-Ala + Arg
-
 ?
3.4.17.12 Leu-Ala-Arg + H2O human anaphylatoxin C3A fragment 75-77 Homo sapiens Leu-Ala + Arg
-
 ?
3.4.17.12 Leu-Gly-Leu-Ala-Arg + H2O human anaphylatoxin C3A fragment 73-77 Homo sapiens Leu-Gly-Leu-Ala + Arg
-
 ?
3.4.17.12 N-(4-hydroxybenzoyl)-Gly-Arg + H2O
-
 Homo sapiens N-(4-hydroxybenzoyl)-Gly + L-arginine
-
 ?
3.4.17.12 Pro-Gly-Lys-Ala-Arg + H2O
-
 Homo sapiens Pro-Gly-Lys-Ala + Arg
-
 ?
3.4.17.12 Ser-His-Leu-Gly-Leu-Ala-Arg + H2O human anaphylatoxin C3A fragment 71-77 Homo sapiens Ser-His-Leu-Gly-Leu-Ala + Arg
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.17.12 carboxypeptidase M
-
 Homo sapiens
3.4.17.12 CPM
-
 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.17.12 37
-
 enzyme assay Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.17.12 1
-
 benzoyl-Ile-Arg CPMwt, pH 7.4, 37°C Homo sapiens
3.4.17.12 1.9
-
 Gly-Gly-Arg CPMwt, pH 7.2, 37°C Homo sapiens
3.4.17.12 3.9
-
 benzoyl-Gly-Arg CPMwt, pH 7.4, 37°C Homo sapiens
3.4.17.12 4.2
-
 Leu-Ala-Arg CPMwt, pH 7.4, 37°C Homo sapiens
3.4.17.12 4.9
-
 N-(4-hydroxybenzoyl)-Gly-Arg CPMwt, pH 7.2, 37°C Homo sapiens
3.4.17.12 5.7
-
 benzoyl-Gly-Arg CPMwt, pH 7.2, 37°C Homo sapiens
3.4.17.12 5.7
-
 N-(4-hydroxybenzoyl)-Gly-Arg hCPM, pH 7.2, 37°C Homo sapiens
3.4.17.12 6.07
-
 benzoyl-Gly-Arg hCPM, pH 7.2, 37°C Homo sapiens
3.4.17.12 6.1
-
 Leu-Gly-Leu-Ala-Arg CPMwt, pH 7.4, 37°C Homo sapiens
3.4.17.12 6.6
-
 His-Leu-Gly-Leu-Ala-Arg CPMwt, pH 7.4, 37°C Homo sapiens
3.4.17.12 6.7
-
 Ser-His-Leu-Gly-Leu-Ala-Arg CPMwt, pH 7.4, 37°C Homo sapiens
3.4.17.12 7.4
-
 Ala-Ser-His-Leu-Gly-Leu-Ala-Arg CPMwt, pH 7.4, 37°C Homo sapiens
3.4.17.12 7.7
-
 benzoyl-Val-Arg CPMwt, pH 7.4, 37°C Homo sapiens
3.4.17.12 8.2
-
 Gly-Leu-Ala-Arg CPMwt, pH 7.4, 37°C Homo sapiens
3.4.17.12 9.6
-
 Benzoyl-Gly-Lys hCPM, pH 7.2, 37°C Homo sapiens
3.4.17.12 11
-
 Benzoyl-Gly-Lys CPMwt, pH 7.2, 37°C Homo sapiens
3.4.17.12 11
-
 furylacryloyl-Ala-Lys CPMwt, pH 7.2, 37°C Homo sapiens
3.4.17.12 11
-
 Pro-Gly-Lys-Ala-Arg CPMwt, pH 7.2, 37°C Homo sapiens
3.4.17.12 14
-
 benzoyl-His-Arg CPMwt, pH 7.4, 37°C Homo sapiens
3.4.17.12 17
-
 Dansyl-Ala-Arg CPMwt, pH 7.2, 37°C Homo sapiens
3.4.17.12 17.6
-
 Dansyl-Ala-Arg hCPM, pH 7.2, 37°C Homo sapiens
3.4.17.12 19
-
 benzoyl-Leu-Arg CPMwt, pH 7.4, 37°C Homo sapiens
3.4.17.12 22
-
 benzoyl-Gln-Arg CPMwt, pH 7.4, 37°C Homo sapiens
3.4.17.12 22.9
-
 benzoyl-Thr-Arg CPMwt, pH 7.4, 37°C Homo sapiens
3.4.17.12 23
-
 benzoyl-Trp-Arg CPMwt, pH 7.4, 37°C Homo sapiens
3.4.17.12 27
-
 benzoyl-Tyr-Arg CPMwt, pH 7.4, 37°C Homo sapiens
3.4.17.12 30
-
 bradykinin(-Phe-Arg) CPMwt, pH 7.2, 37°C Homo sapiens
3.4.17.12 32
-
 furylacryloyl-Ala-Arg hCPM, pH 7.2, 37°C Homo sapiens
3.4.17.12 33
-
 benzoyl-Phe-Arg CPMwt, pH 7.4, 37°C Homo sapiens
3.4.17.12 35
-
 benzoyl-Asn-Arg CPMwt, pH 7.4, 37°C Homo sapiens
3.4.17.12 40
-
 furylacryloyl-Ala-Arg CPMwt, pH 7.2, 37°C Homo sapiens
3.4.17.12 60
-
 benzoyl-Ser-Arg CPMwt, pH 7.4, 37°C Homo sapiens
3.4.17.12 77
-
 benzoyl-Ala-Arg CPMwt, pH 7.4, 37°C Homo sapiens
3.4.17.12 87
-
 benzoyl-Met-Arg CPMwt, pH 7.4, 37°C Homo sapiens
3.4.17.12 88
-
 benzoyl-Ala-Arg hCPM, pH 7.2, 37°C Homo sapiens
3.4.17.12 107
-
 benzoyl-Ala-Arg CPMwt, pH 7.2, 37°C Homo sapiens
3.4.17.12 112
-
 benzoyl-Met-Arg hCPM, pH 7.2, 37°C Homo sapiens
3.4.17.12 130
-
 benzoyl-Met-Arg CPMwt, pH 7.2, 37°C Homo sapiens
3.4.17.12 273
-
 benzoyl-Gly-argininic acid hCPM, pH 7.2, 37°C Homo sapiens
3.4.17.12 316
-
 benzoyl-Gly-argininic acid CPMwt, pH 7.2, 37°C Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.17.12 7.2
-
 enzyme assay Homo sapiens

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.4.17.12 0.000003
-
 MERGETPA CPMwt Homo sapiens
3.4.17.12 0.000006
-
 MERGETPA hCPM Homo sapiens