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Literature summary extracted from

  • Uraji, M.; Arima, J.; Uesugi, Y.; Iwabuchi, M.; Hatanaka, T.
    Effect of salt on the activity of Streptomyces prolyl aminopeptidase (2007), Biochim. Biophys. Acta, 1774, 1462-1469.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.11.5 expression in Escherichia coli Streptomyces lividans
3.4.11.5 expression in Escherichia coli. Enzyme carrying a C-terminal His-tag is less stable than native enzyme Kitasatospora aureofaciens

Protein Variants

EC Number Protein Variants Comment Organism
3.4.11.5 additional information construction of chimera between the salt-sensitive enzyme from Streptomyces lividans and the salt-resistant enzyme from Streptomyces aureofaciens. The fine tuning of the N-terminal conformation of Streptomyces aureofaciens enzyme by hydrophobic interaction is important for the salt tolerance Kitasatospora aureofaciens
3.4.11.5 additional information construction of chimera between the salt-sensitive enzyme from Streptomyces lividans and the salt-resistant enzyme from Streptomyces aureofaciens. The fine tuning of the N-terminal conformation of Streptomyces aureofaciens enzyme by hydrophobic interaction is important for the salt tolerance Streptomyces lividans

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.11.5 additional information enzyme is still active in 4.0 M NaCl Kitasatospora aureofaciens
3.4.11.5 p-chloromercuribenzoic acid
-
 Kitasatospora aureofaciens
3.4.11.5 pefabloc
-
 Kitasatospora aureofaciens
3.4.11.5 phenylmethylsulfonyl fluoride
-
 Kitasatospora aureofaciens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.11.5 42000
-
 6 * 42000, SDS-PAGE, 6 * 47519, calculated Kitasatospora aureofaciens
3.4.11.5 47519
-
 6 * 42000, SDS-PAGE, 6 * 47519, calculated Kitasatospora aureofaciens
3.4.11.5 250000
-
 gel filtration Kitasatospora aureofaciens

Organism

EC Number Organism UniProt Comment Textmining
3.4.11.5 Kitasatospora aureofaciens
-
-
-
3.4.11.5 Kitasatospora aureofaciens TH-3
-
-
-
3.4.11.5 Streptomyces lividans
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.11.5 culture fluid
-
 Kitasatospora aureofaciens
-
3.4.11.5 culture fluid
-
 Streptomyces lividans
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.11.5 3.28
-
 pH 7.0, 30°C Kitasatospora aureofaciens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.11.5 L-Pro-4-nitroanilide + H2O
-
 Streptomyces lividans L-Pro + 4-nitroaniline
-
 ?
3.4.11.5 L-Pro-4-nitroanilide + H2O highly selective Kitasatospora aureofaciens L-Pro + 4-nitroaniline
-
 ?
3.4.11.5 L-Pro-4-nitroanilide + H2O highly selective Kitasatospora aureofaciens TH-3 L-Pro + 4-nitroaniline
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.11.5 hexamer 6 * 42000, SDS-PAGE, 6 * 47519, calculated Kitasatospora aureofaciens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.11.5 55
-
-
 Kitasatospora aureofaciens

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.4.11.5 50
-
 up to Kitasatospora aureofaciens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.11.5 7 7.8
-
 Kitasatospora aureofaciens

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.4.11.5 5 9
-
 Kitasatospora aureofaciens