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Literature summary extracted from

  • Rezaie, A.R.; Sun, M.F.; Gailani, D.
    Contributions of basic amino acids in the autolysis loop of factor XIa to serpin specificity (2006), Biochemistry, 45, 9427-9433.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.21.27 expression of wild-type and mutant enzymes in HEK-293 cells and secretion of recombinant protein to the medium Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
3.4.21.27 K145A site-directed mutagenesis of an autolysis loop residue, the mutant shows altered sensitivity to inhibition by serpins compared to the wild-type enzyme Homo sapiens
3.4.21.27 K148A site-directed mutagenesis of an autolysis loop residue, the mutant shows altered sensitivity to inhibition by serpins compared to the wild-type enzyme Homo sapiens
3.4.21.27 K149A site-directed mutagenesis of an autolysis loop residue, the mutant shows altered sensitivity to inhibition by serpins compared to the wild-type enzyme Homo sapiens
3.4.21.27 R144A site-directed mutagenesis of an autolysis loop residue, the mutant shows altered sensitivity to inhibition by serpins compared to the wild-type enzyme Homo sapiens
3.4.21.27 R144A/K145A/R147A/K148A/K149A site-directed mutagenesis of an autolysis loop residues, the mutant shows altered sensitivity to inhibition by serpins compared to the wild-type enzyme Homo sapiens
3.4.21.27 R147A site-directed mutagenesis of an autolysis loop residue, the mutant shows altered sensitivity to inhibition by serpins compared to the wild-type enzyme Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.21.27 alpha1-protease inhibitor
-
 Homo sapiens
3.4.21.27 antithrombin
-
 Homo sapiens
3.4.21.27 C1-inhibitor
-
 Homo sapiens
3.4.21.27 protein Z dependent protease inhibitor
-
 Homo sapiens
3.4.21.27 serpins inhibition of autolysis of fXIa Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.21.27 extracellular
-
 Homo sapiens
-
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.21.27 factor IX + H2O Homo sapiens
-
 activated factor IX + ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.27 Homo sapiens P03951
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.4.21.27 proteolytic modification the enzyme shows activation by autolysis involving residues of the autolysis loops, overview Homo sapiens

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.21.27 recombinant wild-type and mutant enzymes from HEK-293 cells by immunoaffinity, cation exchange, and heparin affinity chromatography Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.21.27 plasma
-
 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.21.27 factor IX + H2O
-
 Homo sapiens activated factor IX + ?
-
 ?
3.4.21.27 S-2366 + H2O commercial chromogenic substrate Homo sapiens ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.21.27 factor XIa
-
 Homo sapiens
3.4.21.27 FXIa
-
 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.21.27 37
-
 assay at Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.21.27 7.4
-
 assay at Homo sapiens

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.4.21.27 additional information
-
 additional information second-order inhibition rate constants, wild-type and mutant enzymes with different inhibitors, overview Homo sapiens