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Literature summary extracted from

  • Beaufort, N.; Wojciechowski, P.; Sommerhoff, C.P.; Szmyd, G.; Dubin, G.; Eick, S.; Kellermann, J.; Schmitt, M.; Potempa, J.; Magdolen, V.
    The human fibrinolytic system is a target for the staphylococcal metalloprotease aureolysin (2008), Biochem. J., 410, 157-165.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.24.29 alpha2-Macroglobulin a regulatory serpin, the initial N-terminal hydrolysis of alpha2-macroglobulin by aureolysin does not affect the serpin inhibitory activity, cleavage within its exposed reactive loop is associated with a decreased inhibitory activity, down to 23% of the control inhibitor Staphylococcus aureus
3.4.24.29 EDTA
-
 Staphylococcus aureus
3.4.24.29 plasminogen activator inhibitor-1 PAI-1, a regulatory serpin, interaction analysis with aureolysin, overview, the proteolytic degradation of PAI-1 by aureolysin is associated with a drastic decrease in its capacity to inhibit uPA, down to 7% of the inhibitory activity of the control PAI-1 Staphylococcus aureus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.24.29 additional information
-
 additional information kinetics Staphylococcus aureus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.24.29 extracellular aureolysin is secreted Staphylococcus aureus
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.24.29 Ca2+
-
 Staphylococcus aureus

Organism

EC Number Organism UniProt Comment Textmining
3.4.24.29 Staphylococcus aureus
-
 wild-type strains 8325-4 and Newman, and clinical isolates
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.24.29 alpha2-macroglobulin + H2O processing of the inhibitor, the initial N-terminal hydrolysis of alpha2-macroglobulin by aureolysin does not affect the serpin inhibitory activity, cleavage within its exposed reactive loop is associated with a decreased inhibitory activity, down to 23% of the control inhibitor Staphylococcus aureus ?
-
 ?
3.4.24.29 Gelatin + H2O
-
 Staphylococcus aureus ?
-
 ?
3.4.24.29 plasminogen + H2O
-
 Staphylococcus aureus angiostatin + mini-plasminogen
-
 ?
3.4.24.29 plasminogen activator inhibitor-1 + H2O processing of the inhibitor, the proteolytic degradation of PAI-1 by aureolysin is associated with a drastic decrease in its capacity to inhibit uPA, down to 7% of the inhibitory activity of the control PAI-1 Staphylococcus aureus ?
-
 ?
3.4.24.29 pro-urokinase-type plasmin activator + H2O human substrate, activation by cleavage into two enzyme chains, activity by wild-type strains 8325-4 and Newman, and clinical isolates, overview, no activity with N-terminal enzyme substrate mutants, overview Staphylococcus aureus 2 chains of urokinase-type plasmin activator
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.24.29 Aur
-
 Staphylococcus aureus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.24.29 37
-
 assay at Staphylococcus aureus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.24.29 7.4 7.8 assay at Staphylococcus aureus