Literature summary extracted from
Rea, D.; Hazell, C.; Andrews, N.W.; Morty, R.E.; Fueloep, V.
Expression, purification and preliminary crystallographic analysis of oligopeptidase B from Trypanosoma brucei (2006), Acta Crystallogr. Sect. F, 62, 808-810.
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
3.4.21.83 |
Oligopeptidase B is overexpressed in Escherichia coli as an N-terminally hexahistidine-tagged fusion protein |
Trypanosoma brucei |
Crystallization (Commentary)
EC Number |
Crystallization (Comment) |
Organism |
---|
3.4.21.83 |
using the hanging-drop vapour-diffusion technique in 7% (w/v) polyethylene glycol 6000, 1 M LiCl, 0.1 M bis-tris propane pH 7.5. Diffraction data to 2.7 A resolution are collected using synchrotron radiation. The crystals belong to space group P3121 or P3221, with unit-cell parameters a = b = 124.5, c = 249.9 A. A complete data set to 2.7 A is collected using synchrotron radiation |
Trypanosoma brucei |
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
3.4.21.83 |
Trypanosoma brucei |
- |
- |
- |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
3.4.21.83 |
purified by using metal-affinity chromatography |
Trypanosoma brucei |
Synonyms
EC Number |
Synonyms |
Comment |
Organism |
---|
3.4.21.83 |
oligopeptidase B |
- |
Trypanosoma brucei |