EC Number | Cloned (Comment) | Organism |
---|---|---|
3.4.21.B39 | expressed in Escherichia coli strain BL21 | Mus musculus |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.4.21.B39 | antipain | 13% inhibition at 0.2 mM | Mus musculus | |
3.4.21.B39 | Aprotinin | 34% inhibition at 0.1 g/ml | Mus musculus | |
3.4.21.B39 | diisopropylfluorophosphate | 87% inhibition at 2 mM | Mus musculus | |
3.4.21.B39 | E-64 | 12% inhibition at 0.5 mM | Mus musculus | |
3.4.21.B39 | leupeptin | 70% inhibition at 0.1 mM | Mus musculus | |
3.4.21.B39 | additional information | not inhibited by soybean trypsin inhibitor, benzamidine, pepstatin, EDTA, and chymostatin | Mus musculus | |
3.4.21.B39 | N-alpha-p-tosyl-L-leucine-chloromethylketone | 14% inhibition at 0.2 mM | Mus musculus | |
3.4.21.B39 | N-alpha-p-tosyl-L-phenylalanine-chloromethylketone | 14% inhibition at 0.2 mM | Mus musculus | |
3.4.21.B39 | o-phenanthroline | 10% inhibition at 2 mM | Mus musculus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.4.21.B39 | 0.03 | - |
diglutamyl-Gly-Arg-7-amido-4-methylcoumarin | in 0.1 M Tris-HCl buffer (pH 8.0) | Mus musculus | |
3.4.21.B39 | 0.03 | - |
diglutamyl-Pro-Phe-Arg-7-amido-4-methylcoumarin | in 0.1 M Tris-HCl buffer (pH 8.0) | Mus musculus | |
3.4.21.B39 | 0.05 | - |
benzyloxycarbonyl-Leu-Arg-7-amido-4-methylcoumarin | in 0.1 M Tris-HCl buffer (pH 8.0) | Mus musculus | |
3.4.21.B39 | 0.11 | - |
benzyloxycarbonyl-Val-Val-Arg-7-amido-4-methylcoumarin | in 0.1 M Tris-HCl buffer (pH 8.0) | Mus musculus | |
3.4.21.B39 | 0.13 | - |
benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin | in 0.1 M Tris-HCl buffer (pH 8.0) | Mus musculus |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.4.21.B39 | 25500 | - |
non-reducing SDS-PAGE | Mus musculus |
3.4.21.B39 | 28500 | - |
reducing SDS-PAGE | Mus musculus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.4.21.B39 | Mus musculus | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.4.21.B39 | Ni2+-chelate column chromatography and Resource Q column chromatography | Mus musculus |
EC Number | Storage Stability | Organism |
---|---|---|
3.4.21.B39 | -20°C, 2 months, no loss of activity | Mus musculus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.4.21.B39 | benzyloxycarbonyl-Leu-Arg-7-amido-4-methylcoumarin + H2O | - |
Mus musculus | benzyloxycarbonyl-Leu-Arg + 7-amino-4-methylcoumarin | - |
? | |
3.4.21.B39 | benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin + H2O | - |
Mus musculus | benzyloxycarbonyl-Phe-Arg + 7-amino-4-methylcoumarin | - |
? | |
3.4.21.B39 | benzyloxycarbonyl-Val-Val-Arg-7-amido-4-methylcoumarin + H2O | - |
Mus musculus | benzyloxycarbonyl-Val-Val-Arg + 7-amino-4-methylcoumarin | - |
? | |
3.4.21.B39 | collagen type IV + H2O | - |
Mus musculus | ? | - |
? | |
3.4.21.B39 | diglutamyl-Gly-Arg-7-amido-4-methylcoumarin + H2O | - |
Mus musculus | diglutamyl-Gly-Arg + 7-amino-4-methylcoumarin | - |
? | |
3.4.21.B39 | diglutamyl-Pro-Phe-Arg-7-amido-4-methylcoumarin + H2O | - |
Mus musculus | diglutamyl-Pro-Phe-Arg + 7-amino-4-methylcoumarin | - |
? | |
3.4.21.B39 | Fibronectin + H2O | - |
Mus musculus | ? | - |
? | |
3.4.21.B39 | Gelatin + H2O | - |
Mus musculus | ? | - |
? | |
3.4.21.B39 | high-molecular-weight kininogen + H2O | - |
Mus musculus | ? | - |
? | |
3.4.21.B39 | insulin-like growth factor-binding protein-3 + H2O | - |
Mus musculus | ? | - |
? | |
3.4.21.B39 | additional information | no activity with tert-butyloxycarbonyl-Val-Leu-Lys-7-amido-4-methylcoumarin, tert-butyloxycarbonyl-Glu-Lys-Lys-7-amido-4-methylcoumarin, acetyl-Ile-Glu-Thr-Asp-7-amido-4-methylcoumarin, succinyl-Ala-Ala-Pro-Phe-7-amido-4-methylcoumarin, succinyl-Leu-Leu-Val-Tyr-7-amido-4-methylcoumarin, casein, laminin, and single-chain tissue-type plasminogen activator | Mus musculus | ? | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.4.21.B39 | tissue kallikrein 5 | serine protease with trypsin-like activity for Arg at the P1 position | Mus musculus |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.4.21.B39 | 0.03 | - |
diglutamyl-Gly-Arg-7-amido-4-methylcoumarin | in 0.1 M Tris-HCl buffer (pH 8.0) | Mus musculus | |
3.4.21.B39 | 0.03 | - |
diglutamyl-Pro-Phe-Arg-7-amido-4-methylcoumarin | in 0.1 M Tris-HCl buffer (pH 8.0) | Mus musculus | |
3.4.21.B39 | 0.05 | - |
benzyloxycarbonyl-Leu-Arg-7-amido-4-methylcoumarin | in 0.1 M Tris-HCl buffer (pH 8.0) | Mus musculus | |
3.4.21.B39 | 0.11 | - |
benzyloxycarbonyl-Val-Val-Arg-7-amido-4-methylcoumarin | in 0.1 M Tris-HCl buffer (pH 8.0) | Mus musculus | |
3.4.21.B39 | 0.13 | - |
benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin | in 0.1 M Tris-HCl buffer (pH 8.0) | Mus musculus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.4.21.B39 | 9.5 | - |
- |
Mus musculus |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
3.4.21.B39 | 7 | 10 | - |
Mus musculus |