Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Li, Y.N.; Meng, K.; Wang, Y.R.; Yao, B.
    A beta-mannanase from Bacillus subtilis B36: purification, properties, sequencing, gene cloning and expression in Escherichia coli (2007), Z. Naturforsch. C, 61, 840-846.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.78 expression in Escherichia coli Bacillus subtilis

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.78 Ag+ 1 mM, 3% residual activity Bacillus subtilis
3.2.1.78 Hg2+ 1 mM, 2% residual activity Bacillus subtilis
3.2.1.78 additional information not inhibitory: EDTA, 2-mercaptoethanol Bacillus subtilis
3.2.1.78 sodium dodecylsulfate 1 mM, 79% residual activity Bacillus subtilis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.78 extracellular
-
 Bacillus subtilis
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.78 38000
-
 x * 38013, calculated, x * 38000, SDS-PAGE, mature protein Bacillus subtilis
3.2.1.78 38013
-
 x * 38013, calculated, x * 38000, SDS-PAGE, mature protein Bacillus subtilis

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.78 Bacillus subtilis
-
-
-
3.2.1.78 Bacillus subtilis B36
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.78 from culture supernatant Bacillus subtilis

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.78 culture fluid
-
 Bacillus subtilis
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.78 928
-
 pH 6.4, 50°C Bacillus subtilis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.78 guar gum + H2O 21% of the activity with locust bean gum Bacillus subtilis ?
-
 ?
3.2.1.78 guar gum + H2O 21% of the activity with locust bean gum Bacillus subtilis B36 ?
-
 ?
3.2.1.78 locust bean gum + H2O enzyme is highly specific for substrate Bacillus subtilis ?
-
 ?
3.2.1.78 locust bean gum + H2O enzyme is highly specific for substrate Bacillus subtilis B36 ?
-
 ?
3.2.1.78 additional information no substrate: carboxymethyl cellulose Bacillus subtilis ?
-
 ?
3.2.1.78 additional information no substrate: carboxymethyl cellulose Bacillus subtilis B36 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.78 ? x * 38013, calculated, x * 38000, SDS-PAGE, mature protein Bacillus subtilis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.78 50
-
-
 Bacillus subtilis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.78 60
-
 60 min, 90% residual activity Bacillus subtilis
3.2.1.78 70
-
 30 min, 20% residual activity Bacillus subtilis
3.2.1.78 80
-
 20 min, 18% residual activity, 30 min, complete loss of activity Bacillus subtilis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.78 6.4
-
-
 Bacillus subtilis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.78 5 8 more than 70% of maximum activity Bacillus subtilis
3.2.1.78 10
-
 44% of maximum activity Bacillus subtilis