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Literature summary extracted from

  • Kalifa, S.B.; Limam, F.; Smaali, M.I.; Maugard, T.; Marzouki, M.N.
    ?-glucosidase from Sclerotinia sclerotiorum: a new and efficient purification procedure and use as a suitable marker in immuno-enzymatic assay (2007), World J. Microbiol. Biotechnol., 23, 1363-1370.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.21 0.45
-
 4-nitrophenyl-beta-D-glucopyranoside pH 5.0, 55°C Sclerotinia sclerotiorum

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.21 Sclerotinia sclerotiorum
-
 isolated from sunflower Helianthus annuus in Tunisia
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.21 native beta-glu2 60fold to homogeneity by ammonium sulfate precipitation, gel filtration, anion exchange chromatography, and chromatofocusing Sclerotinia sclerotiorum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.21 584
-
 purified beta-glu2 Sclerotinia sclerotiorum

Storage Stability

EC Number Storage Stability Organism
3.2.1.21 25°C, 1% SDS or urea, 1 week, 60% remaining activity Sclerotinia sclerotiorum
3.2.1.21 25°C, 1% Tween 20 or Triton X-100, 1 week, 90% remaining activity Sclerotinia sclerotiorum
3.2.1.21 90% of beta-Glu2 activity is retained when the enzyme is incubated for 6 days in the presence of 5 mg of BSA/ml or non-specific IgG Sclerotinia sclerotiorum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.21 4-nitrophenyl beta-D-glucopyranoside + H2O
-
 Sclerotinia sclerotiorum 4-nitrophenol + beta-D-glucose
-
 ?
3.2.1.21 4-nitrophenyl beta-D-xylopyranoside + H2O
-
 Sclerotinia sclerotiorum 4-nitrophenol + beta-D-xylose
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.21 beta-glu2
-
 Sclerotinia sclerotiorum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.21 55
-
 assay at Sclerotinia sclerotiorum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.21 55
-
 purified enzyme, half-life is 85 min Sclerotinia sclerotiorum
3.2.1.21 65
-
 purified enzyme, half-life is 25 min Sclerotinia sclerotiorum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.21 5
-
 assay at Sclerotinia sclerotiorum

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.2.1.21 Sclerotinia sclerotiorum polybuffer exchanger PBE 94 TM chromatofocusing
-
 4.45