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Literature summary extracted from

  • Rock, C.O.; Cronan, J.E.
    Acyl-acyl carrier protein synthetase from Escherichia coli (1981), Methods Enzymol., 71, 163-168.
    View publication on PubMed

Application

EC Number Application Comment Organism
6.2.1.20 synthesis the enzyme is a useful tool for the synthesis of native acyl-[acyl-carrier-protein] for use in examining other lipid biosynthetic enzymes in E. coli Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.2.1.20 0.005 0.015 saturated fatty acids C12:0 to C16:0 Escherichia coli
6.2.1.20 0.03 0.16 unsaturated fatty acids fatty acids with shorter chain length than 12 Escherichia coli

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
6.2.1.20 inner membrane
-
 Escherichia coli
-
-
6.2.1.20 membrane
-
 Escherichia coli 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.2.1.20 Mg2+ required Escherichia coli
6.2.1.20 Salt requirement for high concentrations of salt, with the exception of Mg2+, the requirement for ion is nonspecific. Chaotrophic salts are effective at lower concentrations than less chaotrophic salts Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.2.1.20 additional information Escherichia coli enzyme may function to reintroduce free fatty acids into the biosynthetic pathway ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.2.1.20 Escherichia coli
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.2.1.20 ATP + an acid + [acyl-carrier protein] the best substrates are saturated fatty acids, C12:0 to C16:0 Escherichia coli AMP + diphosphate + acyl-[acyl-carrier protein]
-
 ?
6.2.1.20 ATP + palmitic acid + [acyl-carrier protein]
-
 Escherichia coli AMP + diphosphate + palmitoyl-[acyl-carrier protein]
-
 ?
6.2.1.20 ATP + saturated fatty acids + an [acyl-carrier protein]
-
 Escherichia coli ?
-
 ?
6.2.1.20 ATP + unsaturated fatty acids + an [acyl-carrier protein]
-
 Escherichia coli ?
-
 ?
6.2.1.20 additional information enzyme may function to reintroduce free fatty acids into the biosynthetic pathway Escherichia coli ?
-
 ?