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Literature summary extracted from

  • Petersen, M.; Daniel, R.
    Purification and characterization of an extracellular lipase from Clostridium tetanomorphum (2006), World J. Microbiol. Biotechnol., 22, 431-435.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.1.3 additional information growth medium containing glycerol in addition to fermentable substrates, such as L-glutamate or glucose, induces the extracellular enzyme Clostridium tetanomorphum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.1.3 extracellular
-
 Clostridium tetanomorphum
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.3 70000
-
 native PAGE Clostridium tetanomorphum
3.1.1.3 118000
-
 gel filtration and sedimentation coefficient Clostridium tetanomorphum

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.3 Clostridium tetanomorphum
-
-
-
3.1.1.3 Clostridium tetanomorphum NCTC 543
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.3 native extracellular enzyme 27fold by ammonium sulfate fractionation and anion exchange chromatography, and ultrafiltration to homogeneity Clostridium tetanomorphum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.1.3 additional information growth medium containing glycerol in addition to fermentable substrates, such as L-glutamate or glucose, induces the extracellular enzyme Clostridium tetanomorphum
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.3 900
-
 purified extracellular lipase Clostridium tetanomorphum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.3 additional information with p-nitrophenyl esters of different fatty acids as substrates enzyme activity is highest when the acyl chain is short e.g. C2 Clostridium tetanomorphum ?
-
 ?
3.1.1.3 additional information with p-nitrophenyl esters of different fatty acids as substrates enzyme activity is highest when the acyl chain is short e.g. C2 Clostridium tetanomorphum NCTC 543 ?
-
 ?
3.1.1.3 tributyrin + H2O
-
 Clostridium tetanomorphum dibutyrin + butyrate
-
 ?
3.1.1.3 tributyrin + H2O
-
 Clostridium tetanomorphum NCTC 543 dibutyrin + butyrate
-
 ?
3.1.1.3 tricaprin + H2O
-
 Clostridium tetanomorphum dicaprin + caprate
-
 ?
3.1.1.3 tricaproin + H2O
-
 Clostridium tetanomorphum dicaproin + caproate
-
 ?
3.1.1.3 tricaprylin + H2O
-
 Clostridium tetanomorphum dicaprylin + caprylate
-
 ?
3.1.1.3 trilaurin + H2O
-
 Clostridium tetanomorphum dilaurin + laurate
-
 ?
3.1.1.3 trilaurin + H2O
-
 Clostridium tetanomorphum NCTC 543 dilaurin + laurate
-
 ?
3.1.1.3 triolein + H2O
-
 Clostridium tetanomorphum diolein + oleate
-
 ?
3.1.1.3 triolein + H2O
-
 Clostridium tetanomorphum NCTC 543 diolein + oleate
-
 ?
3.1.1.3 tripalmitin + H2O
-
 Clostridium tetanomorphum dipalmitin + palmitate
-
 ?
3.1.1.3 tripalmitin + H2O
-
 Clostridium tetanomorphum NCTC 543 dipalmitin + palmitate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.1.3 extracellular lipase
-
 Clostridium tetanomorphum