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Literature summary extracted from

  • Siwayaprahm, P.; Audtho, M.; Ohmiya, K.; Wiwat, C.
    Purification and characterization of a Bacillus circulans No. 4.1 chitinase expressed in Escherichia coli (2006), World J. Microbiol. Biotechnol., 22, 331-335.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.14 expression in Escherichia coli M15 (pREP4) Niallia circulans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.14 66000
-
 x * 66000, calculated from sequence Niallia circulans

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.14 Niallia circulans
-
 No. 4.1
-
3.2.1.14 Niallia circulans No. 4.1
-
 No. 4.1
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.14
-
 Niallia circulans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.14 4-methylumbelliferyl-beta-D-N,N'-diacetylchitobiose + H2O
-
 Niallia circulans 4-methylumbelliferone + N,N'-diacetylchitobiose
-
 ?
3.2.1.14 4-methylumbelliferyl-beta-D-N,N'-diacetylchitobiose + H2O
-
 Niallia circulans No. 4.1 4-methylumbelliferone + N,N'-diacetylchitobiose
-
 ?
3.2.1.14 carboxymethyl-chitin + H2O
-
 Niallia circulans ?
-
 ?
3.2.1.14 carboxymethyl-chitin + H2O
-
 Niallia circulans No. 4.1 ?
-
 ?
3.2.1.14 chitin + H2O processive chitinase with endo-type activity Niallia circulans N-acetylglucosamine + N-acetylchitobiose
-
 ?
3.2.1.14 chitin + H2O processive chitinase with endo-type activity Niallia circulans No. 4.1 N-acetylglucosamine + N-acetylchitobiose
-
 ?
3.2.1.14 colloidal chitin + H2O
-
 Niallia circulans N-acetyl-D-glucosamine + N,N'-diacetylchitobiose
-
 ?
3.2.1.14 glycol chitin + H2O
-
 Niallia circulans ?
-
 ?
3.2.1.14 glycol chitin + H2O
-
 Niallia circulans No. 4.1 ?
-
 ?
3.2.1.14 additional information when cleaved by a proteolytic enzyme, the 20000 Da product can retain high chitinolytic activity Niallia circulans ?
-
 ?
3.2.1.14 additional information when cleaved by a proteolytic enzyme, the 20000 Da product can retain high chitinolytic activity Niallia circulans No. 4.1 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.14 ? x * 66000, calculated from sequence Niallia circulans

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.14 45
-
-
 Niallia circulans

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.14 50
-
 stable up to Niallia circulans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.14 7
-
-
 Niallia circulans

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.14 5 9 stable pH-range Niallia circulans