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Literature summary extracted from

  • Mukherjee, G.; Banerjee, R.
    Effects of temperature, pH and additives on the activity of tannase produced by a co-culture of Rhizopus oryzae and Aspergillus foetidus (2006), World J. Microbiol. Biotechnol., 22, 207-212.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.1.20 Mg2+ activates Aspergillus foetidus
3.1.1.20 Urea slightly activates the enzyme at 0.5 M, but inhibits at higher concentration, 50% inhibition at 3 M Rhizopus arrhizus
3.1.1.20 Urea slightly activates the enzyme at 0.5 M, but inhibits at higher concentration, 50% inhibition at 3 M Aspergillus foetidus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.20 1,10-phenanthroline
-
 Aspergillus foetidus
3.1.1.20 1,10-phenanthroline
-
 Rhizopus arrhizus
3.1.1.20 2-mercaptoethanol
-
 Aspergillus foetidus
3.1.1.20 2-mercaptoethanol
-
 Rhizopus arrhizus
3.1.1.20 Ba2+
-
 Aspergillus foetidus
3.1.1.20 Ba2+
-
 Rhizopus arrhizus
3.1.1.20 Ca2+
-
 Aspergillus foetidus
3.1.1.20 Ca2+
-
 Rhizopus arrhizus
3.1.1.20 Cu2+
-
 Aspergillus foetidus
3.1.1.20 Cu2+
-
 Rhizopus arrhizus
3.1.1.20 EDTA
-
 Aspergillus foetidus
3.1.1.20 EDTA
-
 Rhizopus arrhizus
3.1.1.20 Fe3+
-
 Aspergillus foetidus
3.1.1.20 Fe3+
-
 Rhizopus arrhizus
3.1.1.20 Hg2+
-
 Aspergillus foetidus
3.1.1.20 Hg2+
-
 Rhizopus arrhizus
3.1.1.20 Mn2+
-
 Aspergillus foetidus
3.1.1.20 Mn2+
-
 Rhizopus arrhizus
3.1.1.20 Triton X-100
-
 Aspergillus foetidus
3.1.1.20 Triton X-100
-
 Rhizopus arrhizus
3.1.1.20 Urea slightly activates the enzyme at 0.5 M, but inhibits at higher concentration, 50% inhibition at 3 M Aspergillus foetidus
3.1.1.20 Urea slightly activates the enzyme at 0.5 M, but inhibits at higher concentration, 50% inhibition at 3 M Rhizopus arrhizus
3.1.1.20 Zn2+
-
 Aspergillus foetidus
3.1.1.20 Zn2+
-
 Rhizopus arrhizus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.20 0.21
-
 Tannic acid pH 5.0, 40°C Rhizopus arrhizus
3.1.1.20 0.21
-
 Tannic acid pH 5.0, 40°C Aspergillus foetidus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.1.20 Mg2+ activates Rhizopus arrhizus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.1.20 tannic acid + H2O Rhizopus arrhizus
-
 gallate + ?
-
 ?
3.1.1.20 tannic acid + H2O Aspergillus foetidus
-
 gallate + ?
-
 ?
3.1.1.20 tannic acid + H2O Rhizopus arrhizus RO IIT RB-13
-
 gallate + ?
-
 ?
3.1.1.20 tannic acid + H2O Aspergillus foetidus GMRB 013 MTCC 3557
-
 gallate + ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.20 Aspergillus foetidus
-
-
-
3.1.1.20 Aspergillus foetidus GMRB 013 MTCC 3557
-
-
-
3.1.1.20 Rhizopus arrhizus
-
-
-
3.1.1.20 Rhizopus arrhizus RO IIT RB-13
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.20 native enzyme by acetone precipitation and anion exchange chromatography Rhizopus arrhizus
3.1.1.20 native enzyme by acetone precipitation and anion exchange chromatography Aspergillus foetidus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.1.20 additional information co-culture with Aspergillus foetidus, solid-state fermentation on tannin rich substrates Rhizopus arrhizus
-
3.1.1.20 additional information co-culture with Rhizopus oryzae, solid-state fermentation on tannin rich substrates Aspergillus foetidus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.20 tannic acid + H2O
-
 Rhizopus arrhizus gallate + ?
-
 ?
3.1.1.20 tannic acid + H2O
-
 Aspergillus foetidus gallate + ?
-
 ?
3.1.1.20 tannic acid + H2O
-
 Rhizopus arrhizus RO IIT RB-13 gallate + ?
-
 ?
3.1.1.20 tannic acid + H2O
-
 Aspergillus foetidus GMRB 013 MTCC 3557 gallate + ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.20 40
-
 enzyme from co-culture with Aspergillus foetidus Rhizopus arrhizus
3.1.1.20 40
-
 enzyme from co-culture with Rhizopus oryzae Aspergillus foetidus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.1.1.20 5 55 activity range Rhizopus arrhizus
3.1.1.20 5 55 activity range Aspergillus foetidus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.1.20 40
-
 inactivation of purified enzyme after 255 min Rhizopus arrhizus
3.1.1.20 40
-
 inactivation of purified enzyme after 255 min Aspergillus foetidus
3.1.1.20 60
-
 inactivation of purified enzyme after 105 min Rhizopus arrhizus
3.1.1.20 60
-
 inactivation of purified enzyme after 105 min Aspergillus foetidus
3.1.1.20 65
-
 inactivation of purified enzyme after 75 min Rhizopus arrhizus
3.1.1.20 65
-
 inactivation of purified enzyme after 75 min Aspergillus foetidus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.20 5
-
 enzyme from co-culture with Aspergillus foetidus, the tannase needs an acidic environment to be active Rhizopus arrhizus
3.1.1.20 5
-
 enzyme from co-culture with Rhizopus oryzae, the tannase needs an acidic environment to be active Aspergillus foetidus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.1.20 3 6 activity range Rhizopus arrhizus
3.1.1.20 3 6 activity range Aspergillus foetidus