EC Number | Cloned (Comment) | Organism |
---|---|---|
1.14.19.6 | expression of DELTA12-fatty acid desaturase genes in Escherichia coli strain BL21 and Saccharomyces cerevisiae strain IN-VSc1 leads to production of an active enzyme which converts 17.876% and 17.604% of oleic acid to linoleic acid, GC-MS detection in vitro and in vivo | Umbelopsis isabellina |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.14.19.6 | 43000 | - |
SDS-PAGE of cell membranes of transformed Escherichia coli | Umbelopsis isabellina |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.14.19.6 | Umbelopsis isabellina | P59668 | - |
- |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.14.19.6 | additional information | - |
optimal condition for expressing DELTA12-fatty acid desaturase in Saccharomyces cerevisiae is 3% galactose induction for 24 h at 15°C | Umbelopsis isabellina |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.19.6 | oleic acid + reduced acceptor + O2 | - |
Umbelopsis isabellina | linoleic acid + acceptor + H2O | - |
? |