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Literature summary extracted from

  • Zhu, H.; Smith, P.; Wang, L.K.; Shuman, S.
    Structure-function analysis of the 3' phosphatase component of T4 polynucleotide kinase/phosphatase (2007), Virology, 366, 126-136.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.3.32 expressed in Escherichia coli BL21 (DE3) cells Tequatrovirus T4

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.1.3.32 hanging drop vapour diffusion method with 100 mM sodium cacodylate (pH 6.6), 12% PEG-8000, 0.2 M ammonium sulfate, 20 mM urea, and 5 mM dithiothreitol, at 23°C Tequatrovirus T4

Protein Variants

EC Number Protein Variants Comment Organism
3.1.3.32 D187A less than 1% activity of the wild type enzyme Tequatrovirus T4
3.1.3.32 D187E less than 1% activity of the wild type enzyme Tequatrovirus T4
3.1.3.32 D187N less than 1% activity of the wild type enzyme Tequatrovirus T4
3.1.3.32 D277A less than 1% activity of the wild type enzyme Tequatrovirus T4
3.1.3.32 D277E retains 93% activity of the wild type enzyme Tequatrovirus T4
3.1.3.32 D277N less than 1% activity of the wild type enzyme Tequatrovirus T4
3.1.3.32 E195A/Y205A retains 22% activity of the wild type enzyme Tequatrovirus T4
3.1.3.32 E219A retains 51% activity of the wild type enzyme Tequatrovirus T4
3.1.3.32 E233A retains 45% activity of the wild type enzyme Tequatrovirus T4
3.1.3.32 E292A/W294A retains 3% activity of the wild type enzyme Tequatrovirus T4
3.1.3.32 G212A retains 37% activity of the wild type enzyme Tequatrovirus T4
3.1.3.32 K198A retains 82% activity of the wild type enzyme Tequatrovirus T4
3.1.3.32 K253A retains 95% activity of the wild type enzyme Tequatrovirus T4
3.1.3.32 K258A less than 1% activity of the wild type enzyme Tequatrovirus T4
3.1.3.32 K258Q less than 1% activity of the wild type enzyme Tequatrovirus T4
3.1.3.32 K258R less than 1% activity of the wild type enzyme Tequatrovirus T4
3.1.3.32 M199A/Y200A retains 19% activity of the wild type enzyme Tequatrovirus T4
3.1.3.32 N190A/M192A retains 18% activity of the wild type enzyme Tequatrovirus T4
3.1.3.32 R229A retains 94% activity of the wild type enzyme Tequatrovirus T4
3.1.3.32 R287A/Q295A retains 2% activity of the wild type enzyme Tequatrovirus T4
3.1.3.32 S211A less than 1% activity of the wild type enzyme Tequatrovirus T4
3.1.3.32 S211T shows 130% activity of the wild type enzyme Tequatrovirus T4
3.1.3.32 S298A/D300A retains 27% activity of the wild type enzyme Tequatrovirus T4
3.1.3.32 T222A retains 94% activity of the wild type enzyme Tequatrovirus T4
3.1.3.32 T251A shows 120% activity of the wild type enzyme Tequatrovirus T4

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.32 Tequatrovirus T4
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.32 Ni2+-nitrilotriacetic acid-agarose chromatography Tequatrovirus T4

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.32 3'-phospho-5'-hydroxy-DNA
-
 Tequatrovirus T4 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.3.32 homotetramer x-ray crystallography Tequatrovirus T4

Synonyms

EC Number Synonyms Comment Organism
3.1.3.32 3' phosphatase
-
 Tequatrovirus T4
3.1.3.32 PNKP
-
 Tequatrovirus T4
3.1.3.32 T4 polynucleotide kinase/phosphatase
-
 Tequatrovirus T4