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Literature summary extracted from
- Evidence for a direct role of the Doa4 deubiquitinating enzyme in protein sorting into the MVB pathway (2007), Traffic, 8, 566-581.
Application
| EC Number | Application | Comment | Organism |
|---|---|---|---|
| 3.4.19.12 | additional information | direct role of Doa4 in multivesicular body sorting pathway that is linked to its catalytic activity. Doa4 mediates a deubiquitination step required for sorting of CPS into the multivesicular body pathway | Saccharomyces cerevisiae |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 3.4.19.12 | C571S | is unable to maintain normal ubiquitin levels. In doa4delta cells expressing C571S, CPS is missorted to the limiting membrane of the vacuole | Saccharomyces cerevisiae |
| 3.4.19.12 | additional information | multivesicular body sorting of Gap1 is defective in a doa4delta mutant, which is not suppressed by restoring the ubiquitin pool. In the doa4delta ypt6delta strain, the pool of monomeric ubiquitin is as reduced as in the single doa4delta mutant | Saccharomyces cerevisiae |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.4.19.12 | endosome | the important function of Doa4 in Gap1 endocytosis operates at the late endosome stage | Saccharomyces cerevisiae | 5768 | - |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.4.19.12 | Saccharomyces cerevisiae | - |
- |
- |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.4.19.12 | additional information | deubiquitination by Doa4 of cargo proteins and/or some components of the multivesicular body sorting machinery is essential to correct sorting of cargoes into the multivesicular body pathway | Saccharomyces cerevisiae | ? | - |
? |  |
| 3.4.19.12 | ubiquitin + H2O | - |
Saccharomyces cerevisiae | ? | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.4.19.12 | Doa4 | - |
Saccharomyces cerevisiae |
| 3.4.19.12 | yeast ubiquitin hydrolase | - |
Saccharomyces cerevisiae |
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