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Literature summary extracted from

  • Santos, L.D.; Santos, K.S.; de Souza, B.M.; Arcuri, H.A.; Cunha-Neto, E.; Castro, F.M.; Kalil, J.E.; Palma, M.S.
    Purification, sequencing and structural characterization of the phospholipase A1 from the venom of the social wasp Polybia paulista (Hymenoptera, Vespidae) (2007), Toxicon, 50, 923-937.
    View publication on PubMed

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.1.32 extracellular
-
 Polybia paulista
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.32 33961
-
 x * 33961, PLA1, mass spectrometry Polybia paulista

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.1.32 phosphatidylcholine + H2O Polybia paulista
-
 2-acylglycerophosphocholine + a carboxylate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.32 Polybia paulista A2VBC4 precursor; a social wasp
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.32 native PLA1 21fold from venom by gel filtration and cation exchange chromatography Polybia paulista

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.1.32 venom
-
 Polybia paulista
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.32 2898
-
 purified enzyme Polybia paulista

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.32 1-oleoyl-2-stearoyl-3-sn-glycerophosphorylcholine + H2O
-
 Polybia paulista ?
-
 ?
3.1.1.32 1-stearoyl-2-oleoyl-3-sn-glycerophosphorylcholine + H2O
-
 Polybia paulista ?
-
 ?
3.1.1.32 phosphatidylcholine + H2O
-
 Polybia paulista 2-acylglycerophosphocholine + a carboxylate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.32 ? x * 33961, PLA1, mass spectrometry Polybia paulista
3.1.1.32 More three-dimensional structure determination and anaylsis, PLA1 shows an alpha/beta fold common to lipases, structure comparison and molecular modeling, overview Polybia paulista

Synonyms

EC Number Synonyms Comment Organism
3.1.1.32 PLA1
-
 Polybia paulista

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.32 37
-
 assay at Polybia paulista

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.32 7.9
-
 assay at Polybia paulista