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Literature summary extracted from

  • Cunha, L.; Kuti, M.; Bishop, D.F.; Mezei, M.; Zeng, L.; Zhou, M.M.; Desnick, R.J.
    Human uroporphyrinogen III synthase: NMR-based mapping of the active site (2007), Proteins, 71, 855-873.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.1.37 expressed in Escherichia coli strain Top10 Homo sapiens
4.2.1.75 expression in Escherichia coli Homo sapiens
4.2.1.75 expression of N-terminally His-tagged enzyme in Escherichia coli strain strain BL21(DE3) Homo sapiens

General Stability

EC Number General Stability Organism
4.2.1.75 10% glycerol stabilizes the purified recombinant enzyme in 20 mM sodium phosphate, pH 7.45, 100 mM NaCl, 2 mM DTT, and 0.1 mM EDTA at -80°C Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
4.2.1.75 cytoplasm
-
Homo sapiens 5737
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.1.37 41070
-
mass spectrometry Homo sapiens
4.1.1.37 41070
-
calculated from amino acid sequence Homo sapiens
4.2.1.75 28712
-
x * 28712, calculated from sequence Homo sapiens
4.2.1.75 28810
-
mass spectrometry Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.1.75 hydroxymethylbilane Homo sapiens the enzyme catalyzes the cyclization and D-ring isomerization of hydroxymethylbilane to uroporphyrinogen III, the cyclic tetrapyrrole and physiologic precursor of heme, chlorophyl, and corrin uroporphyrinogen III + H2O
-
?

Organism

EC Number Organism UniProt Comment Textmining
4.1.1.37 Homo sapiens
-
-
-
4.2.1.75 Homo sapiens P10746
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.1.37 IMAC column chromatography, Mono Q column chromatography, and Superdex 70 gel filtration Homo sapiens
4.2.1.75
-
Homo sapiens
4.2.1.75 recombinant N-terminally His-tagged enzyme from Escherichia coli strain strain BL21(DE3) by ultrafiltration, nickel affinity and anion exchange chromatography, followed by gel filtration to homogeneity Homo sapiens

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.1.1.37 0.75
-
crude enzyme, at 37°C Homo sapiens
4.1.1.37 7.6
-
purified enzyme, at 37°C Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.1.37 uroporphyrinogen I
-
Homo sapiens coproporphyrinogen I + 4 CO2
-
?
4.2.1.75 hydroxymethylbilane
-
Homo sapiens uroporphyrinogen III + H2O
-
?
4.2.1.75 hydroxymethylbilane the enzyme catalyzes the cyclization and D-ring isomerization of hydroxymethylbilane to uroporphyrinogen III, the cyclic tetrapyrrole and physiologic precursor of heme, chlorophyl, and corrin Homo sapiens uroporphyrinogen III + H2O
-
?

Subunits

EC Number Subunits Comment Organism
4.2.1.75 ? x * 28712, calculated from sequence Homo sapiens
4.2.1.75 More mapping of the URO-synthase active site by NMR perturbation studies, modelling by by in silico docking, active site structure, overview Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
4.1.1.37 Uro-decarboxylase
-
Homo sapiens
4.1.1.37 uroporphyrinogen-decarboxylase
-
Homo sapiens
4.2.1.75 URO-synthase
-
Homo sapiens
4.2.1.75 Uroporphyrinogen III synthase
-
Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.1.75 37
-
assay at Homo sapiens

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.2.1.75 30
-
pH 7.4, t1/2: more than 14 days Homo sapiens
4.2.1.75 45
-
pH 7.4, t1/2: 1.9 days Homo sapiens
4.2.1.75 60
-
pH 8.2, t1/2: less than 1 day Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.1.75 8.2
-
-
Homo sapiens
4.2.1.75 8.2
-
assay at Homo sapiens

pH Range

EC Number pH Minimum pH Maximum Comment Organism
4.1.1.37 5 8 in 0.2 M KH2PO4, 0.1 mM dithiothreitol Homo sapiens