EC Number | Cloned (Comment) | Organism |
---|---|---|
4.1.1.37 | expressed in Escherichia coli strain Top10 | Homo sapiens |
4.2.1.75 | expression in Escherichia coli | Homo sapiens |
4.2.1.75 | expression of N-terminally His-tagged enzyme in Escherichia coli strain strain BL21(DE3) | Homo sapiens |
EC Number | General Stability | Organism |
---|---|---|
4.2.1.75 | 10% glycerol stabilizes the purified recombinant enzyme in 20 mM sodium phosphate, pH 7.45, 100 mM NaCl, 2 mM DTT, and 0.1 mM EDTA at -80°C | Homo sapiens |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
4.2.1.75 | cytoplasm | - |
Homo sapiens | 5737 | - |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
4.1.1.37 | 41070 | - |
mass spectrometry | Homo sapiens |
4.1.1.37 | 41070 | - |
calculated from amino acid sequence | Homo sapiens |
4.2.1.75 | 28712 | - |
x * 28712, calculated from sequence | Homo sapiens |
4.2.1.75 | 28810 | - |
mass spectrometry | Homo sapiens |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.2.1.75 | hydroxymethylbilane | Homo sapiens | the enzyme catalyzes the cyclization and D-ring isomerization of hydroxymethylbilane to uroporphyrinogen III, the cyclic tetrapyrrole and physiologic precursor of heme, chlorophyl, and corrin | uroporphyrinogen III + H2O | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
4.1.1.37 | Homo sapiens | - |
- |
- |
4.2.1.75 | Homo sapiens | P10746 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
4.1.1.37 | IMAC column chromatography, Mono Q column chromatography, and Superdex 70 gel filtration | Homo sapiens |
4.2.1.75 | - |
Homo sapiens |
4.2.1.75 | recombinant N-terminally His-tagged enzyme from Escherichia coli strain strain BL21(DE3) by ultrafiltration, nickel affinity and anion exchange chromatography, followed by gel filtration to homogeneity | Homo sapiens |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
4.1.1.37 | 0.75 | - |
crude enzyme, at 37°C | Homo sapiens |
4.1.1.37 | 7.6 | - |
purified enzyme, at 37°C | Homo sapiens |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.1.1.37 | uroporphyrinogen I | - |
Homo sapiens | coproporphyrinogen I + 4 CO2 | - |
? | |
4.2.1.75 | hydroxymethylbilane | - |
Homo sapiens | uroporphyrinogen III + H2O | - |
? | |
4.2.1.75 | hydroxymethylbilane | the enzyme catalyzes the cyclization and D-ring isomerization of hydroxymethylbilane to uroporphyrinogen III, the cyclic tetrapyrrole and physiologic precursor of heme, chlorophyl, and corrin | Homo sapiens | uroporphyrinogen III + H2O | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
4.2.1.75 | ? | x * 28712, calculated from sequence | Homo sapiens |
4.2.1.75 | More | mapping of the URO-synthase active site by NMR perturbation studies, modelling by by in silico docking, active site structure, overview | Homo sapiens |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
4.1.1.37 | Uro-decarboxylase | - |
Homo sapiens |
4.1.1.37 | uroporphyrinogen-decarboxylase | - |
Homo sapiens |
4.2.1.75 | URO-synthase | - |
Homo sapiens |
4.2.1.75 | Uroporphyrinogen III synthase | - |
Homo sapiens |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
4.2.1.75 | 37 | - |
assay at | Homo sapiens |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
4.2.1.75 | 30 | - |
pH 7.4, t1/2: more than 14 days | Homo sapiens |
4.2.1.75 | 45 | - |
pH 7.4, t1/2: 1.9 days | Homo sapiens |
4.2.1.75 | 60 | - |
pH 8.2, t1/2: less than 1 day | Homo sapiens |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
4.2.1.75 | 8.2 | - |
- |
Homo sapiens |
4.2.1.75 | 8.2 | - |
assay at | Homo sapiens |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
4.1.1.37 | 5 | 8 | in 0.2 M KH2PO4, 0.1 mM dithiothreitol | Homo sapiens |