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Literature summary extracted from
- Mutagenesis of the phosphate-binding pocket of KDPG aldolase enhances selectivity for hydrophobic substrates (2007), Protein Sci., 16, 2368-2377.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 4.1.2.14 | expressed in Escherichia coli K-12 cells | Escherichia coli |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 4.1.2.14 | S184A | the mutation decreases the efficiency of KDPG retro-aldol cleavage only modestly | Escherichia coli |
| 4.1.2.14 | S184D | the mutation leads to strongly reduced enzymatic activity | Escherichia coli |
| 4.1.2.14 | S184L | the mutation is located in the substrate-binding pocket, interacts with the phosphate moiety of KDPG, and improves the catalytic efficiency for the synthesis of a precursor for nikkomycin by 40fold | Escherichia coli |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 4.1.2.14 | 0.1 | - |
2-dehydro-3-deoxy-D-gluconate 6-phosphate | wild type enzyme, in 100 mM HEPES, pH 7.5 | Escherichia coli |  |
| 4.1.2.14 | 0.16 | - |
2-dehydro-3-deoxy-D-gluconate 6-phosphate | mutant enzyme S184A, in 100 mM HEPES, pH 7.5 | Escherichia coli | |
| 4.1.2.14 | 0.35 | - |
2-dehydro-3-deoxy-D-gluconate 6-phosphate | mutant enzyme S184L, in 100 mM HEPES, pH 7.5 | Escherichia coli | |
| 4.1.2.14 | 0.95 | - |
2-dehydro-3-deoxy-D-gluconate 6-phosphate | mutant enzyme S184L, in 100 mM HEPES, pH 7.5 | Escherichia coli | |
| 4.1.2.14 | 1.2 | - |
2-dehydro-3-deoxy-D-gluconate 6-phosphate | mutant enzyme S184D, in 100 mM HEPES, pH 7.5 | Escherichia coli | |
| 4.1.2.14 | 1.5 | - |
2-dehydro-3-deoxy-D-gluconate | mutant enzyme S184L, in 100 mM HEPES, pH 7.5 | Escherichia coli | |
| 4.1.2.14 | 1.8 | - |
2-dehydro-3-deoxy-D-gluconate | mutant enzyme S184D, in 100 mM HEPES, pH 7.5 | Escherichia coli | |
| 4.1.2.14 | 1.9 | - |
2-dehydro-3-deoxy-D-gluconate | wild type enzyme, in 100 mM HEPES, pH 7.5 | Escherichia coli | |
| 4.1.2.14 | 2.3 | - |
2-dehydro-3-deoxy-D-gluconate | mutant enzyme S184A, in 100 mM HEPES, pH 7.5 | Escherichia coli | |
| 4.1.2.14 | 3 | - |
2-dehydro-4-hydroxy-4-(2'-pyridyl)butyrate | mutant enzyme S184A, in 100 mM HEPES, pH 7.5 | Escherichia coli | |
| 4.1.2.14 | 3 | - |
2-dehydro-4-hydroxy-4-(2'-pyridyl)butyrate | mutant enzyme S184D, in 100 mM HEPES, pH 7.5 | Escherichia coli | |
| 4.1.2.14 | 3 | - |
2-dehydro-4-hydroxy-4-(2'-pyridyl)butyrate | mutant enzyme S184L, in 100 mM HEPES, pH 7.5 | Escherichia coli | |
| 4.1.2.14 | 26 | - |
2-dehydro-4-hydroxyoctonate | mutant enzyme S184L, in 100 mM HEPES, pH 7.5 | Escherichia coli | |
| 4.1.2.14 | 32 | - |
2-dehydro-4-hydroxy-4-(2'-pyridyl)butyrate | wild type enzyme, in 100 mM HEPES, pH 7.5 | Escherichia coli | |
| 4.1.2.14 | 51 | - |
2-dehydro-4-hydroxyoctonate | mutant enzyme S184D, in 100 mM HEPES, pH 7.5 | Escherichia coli | |
| 4.1.2.14 | 56 | - |
2-dehydro-4-hydroxyoctonate | mutant enzyme S184A, in 100 mM HEPES, pH 7.5 | Escherichia coli | |
| 4.1.2.14 | 150 | - |
2-dehydro-4-hydroxyoctonate | wild type enzyme, in 100 mM HEPES, pH 7.5 | Escherichia coli | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 4.1.2.14 | Escherichia coli | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 4.1.2.14 | Ni2+-charged chelating Sepharose Fast Flow column chromatography | Escherichia coli |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 4.1.2.14 | 4°C, HEPES (100 mM, pH 7.5), NaCl (100 mM), 10% glycerol, at concentrations ranging from 1 to 16 mM, up to 1 year, remains stable | Escherichia coli |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.1.2.14 | 2-dehydro-3-deoxy-D-galactonate 6-phosphate | - |
Escherichia coli | pyruvate + D-glyceraldehyde 3-phosphate | - |
? | |
| 4.1.2.14 | 2-dehydro-3-deoxy-D-gluconate | - |
Escherichia coli | ? | - |
? | |
| 4.1.2.14 | 2-dehydro-3-deoxy-D-gluconate 6-phosphate | - |
Escherichia coli | pyruvate + D-glyceraldehyde 3-phosphate | - |
? | |
| 4.1.2.14 | 2-dehydro-4-hydroxy-4-(2'-pyridyl)butyrate | - |
Escherichia coli | ? | - |
? | |
| 4.1.2.14 | 2-dehydro-4-hydroxyoctonate | - |
Escherichia coli | ? | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 4.1.2.14 | 2-keto-3-deoxy-6-phosphogluconate aldolase | - |
Escherichia coli |
| 4.1.2.14 | KDPG aldolase | - |
Escherichia coli |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 4.1.2.14 | 0.00029 | - |
2-dehydro-3-deoxy-D-gluconate 6-phosphate | mutant enzyme S184D, in 100 mM HEPES, pH 7.5 | Escherichia coli | |
| 4.1.2.14 | 0.0026 | - |
2-dehydro-3-deoxy-D-gluconate 6-phosphate | mutant enzyme S184L, in 100 mM HEPES, pH 7.5 | Escherichia coli | |
| 4.1.2.14 | 0.0063 | - |
2-dehydro-3-deoxy-D-gluconate 6-phosphate | wild type enzyme, in 100 mM HEPES, pH 7.5 | Escherichia coli | |
| 4.1.2.14 | 0.019 | - |
2-dehydro-3-deoxy-D-gluconate | wild type enzyme, in 100 mM HEPES, pH 7.5 | Escherichia coli | |
| 4.1.2.14 | 0.04 | - |
2-dehydro-3-deoxy-D-gluconate | mutant enzyme S184A, in 100 mM HEPES, pH 7.5 | Escherichia coli | |
| 4.1.2.14 | 0.08 | - |
2-dehydro-3-deoxy-D-gluconate | mutant enzyme S184L, in 100 mM HEPES, pH 7.5 | Escherichia coli | |
| 4.1.2.14 | 0.11 | - |
2-dehydro-3-deoxy-D-gluconate | mutant enzyme S184D, in 100 mM HEPES, pH 7.5 | Escherichia coli | |
| 4.1.2.14 | 1.8 | - |
2-dehydro-4-hydroxyoctonate | mutant enzyme S184D, in 100 mM HEPES, pH 7.5 | Escherichia coli | |
| 4.1.2.14 | 2 | - |
2-dehydro-4-hydroxyoctonate | wild type enzyme, in 100 mM HEPES, pH 7.5 | Escherichia coli | |
| 4.1.2.14 | 2.6 | - |
2-dehydro-4-hydroxyoctonate | mutant enzyme S184A, in 100 mM HEPES, pH 7.5 | Escherichia coli | |
| 4.1.2.14 | 2.9 | - |
2-dehydro-4-hydroxyoctonate | mutant enzyme S184L, in 100 mM HEPES, pH 7.5 | Escherichia coli | |
| 4.1.2.14 | 3.2 | - |
2-dehydro-3-deoxy-D-gluconate 6-phosphate | mutant enzyme S184D, in 100 mM HEPES, pH 7.5 | Escherichia coli | |
| 4.1.2.14 | 6 | - |
2-dehydro-4-hydroxy-4-(2'-pyridyl)butyrate | mutant enzyme S184D, in 100 mM HEPES, pH 7.5 | Escherichia coli | |
| 4.1.2.14 | 11 | - |
2-dehydro-4-hydroxy-4-(2'-pyridyl)butyrate | wild type enzyme, in 100 mM HEPES, pH 7.5 | Escherichia coli | |
| 4.1.2.14 | 27 | - |
2-dehydro-4-hydroxy-4-(2'-pyridyl)butyrate | mutant enzyme S184A, in 100 mM HEPES, pH 7.5 | Escherichia coli | |
| 4.1.2.14 | 40 | - |
2-dehydro-4-hydroxy-4-(2'-pyridyl)butyrate | mutant enzyme S184L, in 100 mM HEPES, pH 7.5 | Escherichia coli | |
| 4.1.2.14 | 74 | - |
2-dehydro-3-deoxy-D-gluconate 6-phosphate | mutant enzyme S184L, in 100 mM HEPES, pH 7.5 | Escherichia coli | |
| 4.1.2.14 | 83 | - |
2-dehydro-3-deoxy-D-gluconate 6-phosphate | wild type enzyme, in 100 mM HEPES, pH 7.5 | Escherichia coli | |
| 4.1.2.14 | 97 | - |
2-dehydro-3-deoxy-D-gluconate 6-phosphate | mutant enzyme S184A, in 100 mM HEPES, pH 7.5 | Escherichia coli | |
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