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Literature summary extracted from

  • Miller, D.J.; Shuvalova, L.; Evdokimova, E.; Savchenko, A.; Yakunin, A.F.; Anderson, W.F.
    Structural and biochemical characterization of a novel Mn2+-dependent phosphodiesterase encoded by the yfcE gene (2007), Protein Sci., 16, 1338-1348.
    View publication on PubMedView publication on EuropePMC

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.1.4.1 2.25 A resolution, in presence and absence of sulfate inhibitor. Protein has a beta-sandwich architecture and is a tetramer with two zinc ions per active site Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.4.1 9.74
-
 bis-p-nitrophenyl phosphate pH 8.5, 37°C Escherichia coli
3.1.4.1 18.3
-
 bis-p-nitrophenyl phosphate pH 8.5, 37°C, presence of 0.5 mM phosphate Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.4.1 Mn2+ KM value 0.022 mM Escherichia coli
3.1.4.1 Zn2+ enzyme carries two zinc ions per active site Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.4.1 79400
-
 gel filtration Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
3.1.4.1 Escherichia coli
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.4.1 bis-p-nitrophenyl phosphate + H2O
-
 Escherichia coli p-nitrophenyl phosphate + nitrophenol
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.4.1 tetramer crystallitazion data Escherichia coli

Synonyms

EC Number Synonyms Comment Organism
3.1.4.1 yfcE
-
 Escherichia coli

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.4.1 19.8
-
 bis-p-nitrophenyl phosphate pH 8.5, 37°C Escherichia coli
3.1.4.1 20.3
-
 bis-p-nitrophenyl phosphate pH 8.5, 37°C, presence of 0.5 mM phosphate Escherichia coli