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Literature summary extracted from

  • Rao, K.N.; Kumaran, D.; Seetharaman, J.; Bonanno, J.B.; Burley, S.K.; Swaminathan, S.
    Crystal structure of trehalose-6-phosphate phosphatase-related protein: biochemical and biological implications (2006), Protein Sci., 15, 1735-1744.
    View publication on PubMedView publication on EuropePMC

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.1.3.12 sitting drop vapour diffusion method with 30% PEG 4000, 0.2 M MgCl2, and 0.1 M Tris-HCl (pH 8.5) Thermoplasma acidophilum

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.12 EDTA
-
 Thermoplasma acidophilum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.12 2.7
-
 trehalose 6-phosphate in 50 mM Tris-HCl (pH 8.0), 5 mM dithiothreitol, and 20 mM MgCl2, at 50°C Thermoplasma acidophilum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.3.12 Mg2+ dependent Thermoplasma acidophilum

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.12 Thermoplasma acidophilum
-
-
-
3.1.3.12 Thermoplasma acidophilum TA1209
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.12 Ni-NTA resin column chromatography and S200 gel filtration Thermoplasma acidophilum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.12 trehalose 6-phosphate + H2O
-
 Thermoplasma acidophilum trehalose + phosphate
-
 ?
3.1.3.12 trehalose 6-phosphate + H2O
-
 Thermoplasma acidophilum TA1209 trehalose + phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.3.12 T6PP
-
 Thermoplasma acidophilum
3.1.3.12 trehalose-6-phosphate phosphatase-related protein
-
 Thermoplasma acidophilum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.3.12 10
-
 trehalose 6-phosphate in 50 mM Tris-HCl (pH 8.0), 5 mM dithiothreitol, and 20 mM MgCl2, at 50°C Thermoplasma acidophilum