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Literature summary extracted from

  • Perveen, R.; Rashid, M.H.; Saleem, M.; Khalid, A.M.; Rajoka, M.I.
    Kinetic and thermodynamic properties of an immobilized glucoamylase from a mesophilic fungus, Arachniotus citrinus (2006), Protein Pept. Lett., 13, 665-671.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.2.1.3 biotechnology enzyme immobilization on polyacrylamide gel results in an enzyme with increases thermostability for use in biocatalysis Gymnascella citrina
3.2.1.3 synthesis enzyme immobilization on polyacrylamide gel results in an enzyme with increases thermostability for use in biocatalysis Gymnascella citrina

Protein Variants

EC Number Protein Variants Comment Organism
3.2.1.3 additional information enzyme immobilization on polyacrylamide gel highly decreases the entropy and enthalpy of thermal enzyme deactivation Gymnascella citrina

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.3 additional information
-
 additional information kinetics and thermodynamics of polyacrylamide gel-immobilized purified enzyme Gymnascella citrina
3.2.1.3 0.031
-
 starch immobilized enzyme, pH 5.5, 90°C Gymnascella citrina
3.2.1.3 0.1
-
 starch free enzyme, pH 5.5, 90°C Gymnascella citrina
3.2.1.3 0.12
-
 starch immobilized enzyme, pH 5.5, 70°C Gymnascella citrina
3.2.1.3 0.18
-
 starch free enzyme, pH 5.5, 70°C Gymnascella citrina
3.2.1.3 0.238
-
 starch immobilized enzyme, pH 5.5, 50°C Gymnascella citrina
3.2.1.3 0.333
-
 starch free enzyme, pH 5.5, 50°C Gymnascella citrina

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.3 starch + H2O Gymnascella citrina
-
 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.3 Gymnascella citrina
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.3 native enzyme 63.3fold by ammonium sulfate fractionation, anion exchange and hydrophobic interaction chromatography, followed by another step of anion exchange chromatography to homogeneity Gymnascella citrina

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.3 additional information
-
-
 Gymnascella citrina

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.3 starch + H2O
-
 Gymnascella citrina ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.3 glucoamylase
-
 Gymnascella citrina

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.3 55
-
 free enzyme Gymnascella citrina
3.2.1.3 60
-
 immobilized enzyme Gymnascella citrina

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.3 50 90 temperature profile of free and immobilized enzyme, overview Gymnascella citrina

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.3 60
-
 immobilized enzyme, stable up to, melting temperature of the free enzyme Gymnascella citrina
3.2.1.3 70
-
 melting temperature of the immobilized enzyme Gymnascella citrina

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.3 3.5
-
 starch immobilized enzyme, pH 5.5, 50°C Gymnascella citrina
3.2.1.3 4.12
-
 starch free enzyme, pH 5.5, 50°C Gymnascella citrina

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.3 4.5
-
 free enzyme Gymnascella citrina
3.2.1.3 5.5
-
 immobilized enzyme Gymnascella citrina

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.3 3 11 pH profile of free and immobilized enzyme, overview Gymnascella citrina