Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Wong, D.W.; Robertson, G.H.; Lee, C.C.; Wagschal, K.
    Synergistic action of recombinant alpha-amylase and glucoamylase on the hydrolysis of starch granules (2007), Protein J., 26, 159-164.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.3 expression in Saccharomyces cerevisiae using the yeast phosphoglycerate kinase gene promoter with the original vector signal sequence eliminated, co-expression with a barley alpha-amylase 1 mutant with constitutive secretion in active forms Lentinula edodes

Protein Variants

EC Number Protein Variants Comment Organism
3.2.1.3 additional information co-expressed recombinant barley alpha-amylase 1 mutant and recombinant GLA synergistically enhanced the rate of hydrolysis by about 3fold for corn and wheat starch granules, compared to the sum of the individual activities, exo-endo synergism, reaction ratios, overview Lentinula edodes

General Stability

EC Number General Stability Organism
3.2.1.3 the recombinant enzyme stability is enhanced by 27% by Ca2+ at 0.1 mM Lentinula edodes

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.3 Cu2+ 53% inhibition at 10 mM Lentinula edodes
3.2.1.3 EDTA slight inhibition Lentinula edodes
3.2.1.3 Fe2+ 29% inhibition at 10 mM Lentinula edodes
3.2.1.3 Mg2+ slight inhibition Lentinula edodes

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.3 Ca2+ bound Ca2+ in the enzyme molecule is essential at 50°C Lentinula edodes

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.3 starch + H2O Lentinula edodes
-
 D-glucose + ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.3 Lentinula edodes
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.3 starch + H2O
-
 Lentinula edodes D-glucose + ?
-
 ?
3.2.1.3 starch + H2O soluble corn and wheat starch granules Lentinula edodes D-glucose + ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.3 GLA
-
 Lentinula edodes
3.2.1.3 glucoamylase
-
 Lentinula edodes

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.3 50
-
-
 Lentinula edodes

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.3 25 50
-
 Lentinula edodes

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.3 50
-
 recombinant enzyme, stable up to, stability is enhanced by Ca2+ Lentinula edodes

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.3 4.6
-
-
 Lentinula edodes