EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
3.2.1.52 | chondroitin 4-sulfate | 0.025 mg/ml, 1.19fold activation | Palythoa caribaeorum | |
3.2.1.52 | chondroitin 6-sulfate | 0.025 mg/ml, 1.35fold activation | Palythoa caribaeorum | |
3.2.1.52 | Phenylmethylsulfonylfluoride | 1 mM, 1.46fold activation | Palythoa caribaeorum |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.2.1.52 | D-fructose | 3 mM, 26% inhibition | Palythoa caribaeorum | |
3.2.1.52 | D-mannose | 3 mM, 19% inhibition | Palythoa caribaeorum | |
3.2.1.52 | fucoidan | 0.025 mg/ml, 19% inhibition | Palythoa caribaeorum | |
3.2.1.52 | HgCl2 | 1 mM, complete inhibition | Palythoa caribaeorum | |
3.2.1.52 | iodoacetamide | 1 mM, 16% inhibition | Palythoa caribaeorum | |
3.2.1.52 | lactose | 3 mM, 20% inhibition | Palythoa caribaeorum | |
3.2.1.52 | maltose | 3 mM, 33% inhibition | Palythoa caribaeorum |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.52 | 0.53 | - |
4-nitrophenyl 2-acetamido-2-deoxy-beta-D-glucopyranose | - |
Palythoa caribaeorum |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.2.1.52 | BaCl2 | 1 mM, 1.4fold activation | Palythoa caribaeorum | |
3.2.1.52 | MgCl2 | 1 mM, 1.17fold activation | Palythoa caribaeorum | |
3.2.1.52 | Na2SeO4 | 1 mM, 1.45fold activation | Palythoa caribaeorum |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.2.1.52 | 25000 | - |
x * 25000, SDS-PAGE | Palythoa caribaeorum |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.2.1.52 | Palythoa caribaeorum | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.2.1.52 | - |
Palythoa caribaeorum |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.2.1.52 | additional information | - |
57.9 U/mg. One unit of enzyme activity was defined as the amount of enzyme which released 0.01 absorbance at 405 nm | Palythoa caribaeorum |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.52 | 4-nitrophenyl 2-acetamido-2-deoxy-beta-D-glucopyranose + H2O | - |
Palythoa caribaeorum | 4-nitrophenol + 2-acetamido-2-deoxy-beta-D-glucopyranose | - |
? | |
3.2.1.52 | 4-nitrophenyl-2-(acetamino)-2-deoxy-beta-D-glucopyranoside + H2O | preferentially hydrolyzed | Palythoa caribaeorum | 4-nitrophenol + N-acetyl-D-glucosamine | - |
? | |
3.2.1.52 | additional information | no activity with chitin azure | Palythoa caribaeorum | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.2.1.52 | ? | x * 25000, SDS-PAGE | Palythoa caribaeorum |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.2.1.52 | Pcb-NAHA1 | - |
Palythoa caribaeorum |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.52 | 45 | 60 | - |
Palythoa caribaeorum |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.52 | 37 | 70 | 37°C: about 60% of maximal activity, 70°C: about 25% of maximal activity | Palythoa caribaeorum |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.52 | 0.61 | - |
4-nitrophenyl-2-(acetamino)-2-deoxy-beta-D-glucopyranoside | - |
Palythoa caribaeorum |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.52 | 5 | - |
- |
Palythoa caribaeorum |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.52 | 4 | 8.5 | pH 4.0: about 35% of maximal activity, pH 5.5: about 35% of maximal activity, pH 8.5: about 50% of maximal activity | Palythoa caribaeorum |