EC Number | Cloned (Comment) | Organism |
---|---|---|
3.2.1.8 | expressed in Pichia pastoris strain GS115 | Bacillus licheniformis |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.2.1.8 | 20000 | - |
SDS-PAGE | Bacillus licheniformis |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.2.1.8 | Bacillus licheniformis | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.2.1.8 | ammonium sulfate precipitation and Sephadex G-100 gel filtration | Bacillus licheniformis |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.8 | xylan + H2O | xylan from birchwood and wheat bran insoluble xylan | Bacillus licheniformis | additional information | reaction products are xylose to xylopentaose with xylotriose as the major product | ? | |
3.2.1.8 | xylobiose + H2O | - |
Bacillus licheniformis | ? | - |
? | |
3.2.1.8 | xylohexaose + H2O | - |
Bacillus licheniformis | ? | - |
? | |
3.2.1.8 | xylopentaose + H2O | - |
Bacillus licheniformis | ? | - |
? | |
3.2.1.8 | xylotetraose + H2O | - |
Bacillus licheniformis | ? | - |
? | |
3.2.1.8 | xylotriose + H2O | - |
Bacillus licheniformis | ? | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.2.1.8 | BlxA | - |
Bacillus licheniformis |
3.2.1.8 | xylanase A | - |
Bacillus licheniformis |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.8 | 60 | - |
- |
Bacillus licheniformis |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.8 | 70 | - |
the recombinant enzyme is stable below 70°C, enzyme activity is rapidly lost above 70°C, the residual activity of the recombinant BlxA is 76% at 70°C and pH 6.0 after 2 min | Bacillus licheniformis |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.8 | 6 | - |
- |
Bacillus licheniformis |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.8 | 5 | 8 | - |
Bacillus licheniformis |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.8 | 5 | 9 | over 80% of recombinant BlxA activity is retained after treatment of the enzyme by preincubation over a pH range of 5.0-9.0 for 1 h at 25°C | Bacillus licheniformis |