BRENDA - Enzyme Database

Expression of recombinant Bacillus licheniformis xylanase A in Pichia pastoris and xylooligosaccharides released from xylans by it

Liu, M.Q.; Liu, G.F.; Protein Expr. Purif. 57, 101-107 (2008) View publication on PubMed

Data extracted from this reference:

Cloned(Commentary)
EC Number
Cloned (Commentary)
Organism
3.2.1.8
expressed in Pichia pastoris strain GS115
Bacillus licheniformis
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.2.1.8
20000
-
SDS-PAGE
Bacillus licheniformis
Organism
EC Number
Organism
UniProt
Commentary
Textmining
3.2.1.8
Bacillus licheniformis
-
-
-
Purification (Commentary)
EC Number
Purification (Commentary)
Organism
3.2.1.8
ammonium sulfate precipitation and Sephadex G-100 gel filtration
Bacillus licheniformis
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
3.2.1.8
xylan + H2O
xylan from birchwood and wheat bran insoluble xylan
682685
Bacillus licheniformis
additional information
reaction products are xylose to xylopentaose with xylotriose as the major product
-
-
?
3.2.1.8
xylobiose + H2O
-
682685
Bacillus licheniformis
?
-
-
-
?
3.2.1.8
xylohexaose + H2O
-
682685
Bacillus licheniformis
?
-
-
-
?
3.2.1.8
xylopentaose + H2O
-
682685
Bacillus licheniformis
?
-
-
-
?
3.2.1.8
xylotetraose + H2O
-
682685
Bacillus licheniformis
?
-
-
-
?
3.2.1.8
xylotriose + H2O
-
682685
Bacillus licheniformis
?
-
-
-
?
Synonyms
EC Number
Synonyms
Commentary
Organism
3.2.1.8
BlxA
-
Bacillus licheniformis
3.2.1.8
xylanase A
-
Bacillus licheniformis
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
3.2.1.8
60
-
-
Bacillus licheniformis
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
3.2.1.8
70
-
the recombinant enzyme is stable below 70°C, enzyme activity is rapidly lost above 70°C, the residual activity of the recombinant BlxA is 76% at 70°C and pH 6.0 after 2 min
Bacillus licheniformis
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.2.1.8
6
-
-
Bacillus licheniformis
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
3.2.1.8
5
8
-
Bacillus licheniformis
pH Stability
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
3.2.1.8
5
9
over 80% of recombinant BlxA activity is retained after treatment of the enzyme by preincubation over a pH range of 5.0-9.0 for 1 h at 25°C
Bacillus licheniformis
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
3.2.1.8
expressed in Pichia pastoris strain GS115
Bacillus licheniformis
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.2.1.8
20000
-
SDS-PAGE
Bacillus licheniformis
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.2.1.8
ammonium sulfate precipitation and Sephadex G-100 gel filtration
Bacillus licheniformis
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
3.2.1.8
xylan + H2O
xylan from birchwood and wheat bran insoluble xylan
682685
Bacillus licheniformis
additional information
reaction products are xylose to xylopentaose with xylotriose as the major product
-
-
?
3.2.1.8
xylobiose + H2O
-
682685
Bacillus licheniformis
?
-
-
-
?
3.2.1.8
xylohexaose + H2O
-
682685
Bacillus licheniformis
?
-
-
-
?
3.2.1.8
xylopentaose + H2O
-
682685
Bacillus licheniformis
?
-
-
-
?
3.2.1.8
xylotetraose + H2O
-
682685
Bacillus licheniformis
?
-
-
-
?
3.2.1.8
xylotriose + H2O
-
682685
Bacillus licheniformis
?
-
-
-
?
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
3.2.1.8
60
-
-
Bacillus licheniformis
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
3.2.1.8
70
-
the recombinant enzyme is stable below 70°C, enzyme activity is rapidly lost above 70°C, the residual activity of the recombinant BlxA is 76% at 70°C and pH 6.0 after 2 min
Bacillus licheniformis
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.2.1.8
6
-
-
Bacillus licheniformis
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
3.2.1.8
5
8
-
Bacillus licheniformis
pH Stability (protein specific)
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
3.2.1.8
5
9
over 80% of recombinant BlxA activity is retained after treatment of the enzyme by preincubation over a pH range of 5.0-9.0 for 1 h at 25°C
Bacillus licheniformis