EC Number | Cloned (Comment) | Organism |
---|---|---|
3.5.1.28 | gene amiB, DNA and amino acid sequence determination and analysis, overexpression of the C-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain DH5alpha | Pseudomonas aeruginosa |
3.5.1.28 | overexpression in Escherichia coli | Pseudomonas aeruginosa |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.5.1.28 | additional information | Pseudomonas aeruginosa | the enzyme cleaves the amide linkage between the stem peptides and the lactyl moiety of muramoyl residues in bacterial cell wall heteropolymer peptidoglycan | ? | - |
? | |
3.5.1.28 | peptidoglycan + H2O | Pseudomonas aeruginosa | - |
? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.5.1.28 | Pseudomonas aeruginosa | - |
- |
- |
3.5.1.28 | Pseudomonas aeruginosa | - |
strain PA01, gene amiB | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.5.1.28 | combination of affinity and cation-exchange chromatographies | Pseudomonas aeruginosa |
3.5.1.28 | recombinant C-terminally His-tagged enzyme 38fold from Escherichia coli by nickel affinity and cation exchange chromatography to 98% homogeneity | Pseudomonas aeruginosa |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.5.1.28 | 132 | - |
purified recombinant enzyme | Pseudomonas aeruginosa |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.5.1.28 | additional information | the enzyme cleaves the amide linkage between the stem peptides and the lactyl moiety of muramoyl residues in bacterial cell wall heteropolymer peptidoglycan | Pseudomonas aeruginosa | ? | - |
? | |
3.5.1.28 | peptidoglycan + H2O | - |
Pseudomonas aeruginosa | ? | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.5.1.28 | AmiB | - |
Pseudomonas aeruginosa |
3.5.1.28 | Autolysin | - |
Pseudomonas aeruginosa |
3.5.1.28 | N-acetylmuramoyl-l-alanine amidase B | - |
Pseudomonas aeruginosa |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.5.1.28 | 25 | - |
assay at | Pseudomonas aeruginosa |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.5.1.28 | 6.5 | - |
assay at | Pseudomonas aeruginosa |
EC Number | Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|---|
3.5.1.28 | Pseudomonas aeruginosa | AmiB, sequence calculation | - |
9.79 |