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Literature summary extracted from

  • Scheurwater, E.M.; Pfeffer, J.M.; Clarke, A.J.
    Production and purification of the bacterial autolysin N-acetylmuramoyl-l-alanine amidase B from Pseudomonas aeruginosa (2007), Protein Expr. Purif., 56, 128-137.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.1.28 gene amiB, DNA and amino acid sequence determination and analysis, overexpression of the C-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain DH5alpha Pseudomonas aeruginosa
3.5.1.28 overexpression in Escherichia coli Pseudomonas aeruginosa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.1.28 additional information Pseudomonas aeruginosa the enzyme cleaves the amide linkage between the stem peptides and the lactyl moiety of muramoyl residues in bacterial cell wall heteropolymer peptidoglycan ?
-
?
3.5.1.28 peptidoglycan + H2O Pseudomonas aeruginosa
-
?
-
?

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.28 Pseudomonas aeruginosa
-
-
-
3.5.1.28 Pseudomonas aeruginosa
-
strain PA01, gene amiB
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.28 combination of affinity and cation-exchange chromatographies Pseudomonas aeruginosa
3.5.1.28 recombinant C-terminally His-tagged enzyme 38fold from Escherichia coli by nickel affinity and cation exchange chromatography to 98% homogeneity Pseudomonas aeruginosa

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.1.28 132
-
purified recombinant enzyme Pseudomonas aeruginosa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.28 additional information the enzyme cleaves the amide linkage between the stem peptides and the lactyl moiety of muramoyl residues in bacterial cell wall heteropolymer peptidoglycan Pseudomonas aeruginosa ?
-
?
3.5.1.28 peptidoglycan + H2O
-
Pseudomonas aeruginosa ?
-
?

Synonyms

EC Number Synonyms Comment Organism
3.5.1.28 AmiB
-
Pseudomonas aeruginosa
3.5.1.28 Autolysin
-
Pseudomonas aeruginosa
3.5.1.28 N-acetylmuramoyl-l-alanine amidase B
-
Pseudomonas aeruginosa

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.1.28 25
-
assay at Pseudomonas aeruginosa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.1.28 6.5
-
assay at Pseudomonas aeruginosa

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.5.1.28 Pseudomonas aeruginosa AmiB, sequence calculation
-
9.79