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Literature summary extracted from

  • Mosbah, H.; Sayari, A.; Horchani, H.; Gargouri, Y.
    Involvement of Gly 311 residue on substrate discrimination, pH and temperature dependency of recombinant Staphylococcus xylosus lipase: a study with emulsified substrate (2007), Protein Expr. Purif., 55, 31-39.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.3 expression of wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) Staphylococcus xylosus

Protein Variants

EC Number Protein Variants Comment Organism
3.1.1.3 G311D site-directed mutagenesis, the mutant shows reduced activity and a distinct pH profile compared to the wild-type enzyme Staphylococcus xylosus
3.1.1.3 G311K site-directed mutagenesis, the mutant shows reduced activity and a distinct pH profile compared to the wild-type enzyme Staphylococcus xylosus
3.1.1.3 G311L site-directed mutagenesis, the mutant shows reduced activity but increased pH and thermal stability compared to the wild-type enzyme, the mutant also shows phospholipase activity with phosphatidylcholine in contrast to the wild-type enzyme Staphylococcus xylosus
3.1.1.3 G311W site-directed mutagenesis, inactive mutant Staphylococcus xylosus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.3 4.7
-
 tributyrin pH 8.0, 37°C, recombinant mutant G311D Staphylococcus xylosus
3.1.1.3 5
-
 olive oil pH 8.0, 37°C, recombinant mutant G311D Staphylococcus xylosus
3.1.1.3 13.9
-
 tributyrin pH 8.0, 37°C, recombinant mutant G311K Staphylococcus xylosus
3.1.1.3 18.9
-
 tributyrin pH 8.0, 37°C, recombinant mutant G311L Staphylococcus xylosus
3.1.1.3 20.4
-
 tributyrin pH 8.0, 37°C, recombinant wild-type enzyme Staphylococcus xylosus
3.1.1.3 27.8
-
 olive oil pH 8.0, 37°C, recombinant mutant G311K Staphylococcus xylosus
3.1.1.3 35.7
-
 olive oil pH 8.0, 37°C, recombinant wild-type enzyme Staphylococcus xylosus
3.1.1.3 100
-
 olive oil pH 8.0, 37°C, recombinant mutant G311L Staphylococcus xylosus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.3 45000
-
 x * 45000, recombinant enzyme, SDS-PAGE Staphylococcus xylosus

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.3 Staphylococcus xylosus Q2TPV1
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.3 recombinant wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Staphylococcus xylosus

Reaction

EC Number Reaction Comment Organism Reaction ID
3.1.1.3 triacylglycerol + H2O = diacylglycerol + a carboxylate residue Gly311 is essential for catalytic activity Staphylococcus xylosus
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.3 1.5
-
 purified recombinant mutant G311K, substrate olive oil Staphylococcus xylosus
3.1.1.3 25
-
 purified recombinant mutant G311D, substrate olive oil Staphylococcus xylosus
3.1.1.3 30
-
 purified recombinant mutant G311K, substrate tributyrin Staphylococcus xylosus
3.1.1.3 100
-
 purified recombinant mutant G311D, substrate tributyrin Staphylococcus xylosus
3.1.1.3 220
-
 purified recombinant mutant G311L, substrate olive oil Staphylococcus xylosus
3.1.1.3 280
-
 purified recombinant mutant G311L, substrate tributyrin Staphylococcus xylosus
3.1.1.3 850
-
 purified recombinant wild-type enzyme, substrate olive oil Staphylococcus xylosus
3.1.1.3 1500
-
 purified recombinant wild-type enzyme, substrate tributyrin Staphylococcus xylosus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.3 tributyrin + H2O
-
 Staphylococcus xylosus dibutyrin + butyrate
-
 ?
3.1.1.3 trioctanoin + H2O
-
 Staphylococcus xylosus dioctanoin + octanoate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.3 ? x * 45000, recombinant enzyme, SDS-PAGE Staphylococcus xylosus

Synonyms

EC Number Synonyms Comment Organism
3.1.1.3 lipase
-
 Staphylococcus xylosus
3.1.1.3 SXL
-
 Staphylococcus xylosus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.3 37
-
 assay at Staphylococcus xylosus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.1.1.3 25 65
-
 Staphylococcus xylosus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.1.3 50
-
 purified recombinant mutant G311L shows increased half-life of 280 min, while the mutant G311D and G311K show 30% and 35% remaining activity, respectively, after 30 min at 50°C Staphylococcus xylosus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.1.3 10
-
 olive oil pH 8.0, 37°C, recombinant mutant G311K Staphylococcus xylosus
3.1.1.3 30
-
 tributyrin pH 8.0, 37°C, recombinant mutant G311K Staphylococcus xylosus
3.1.1.3 37.9
-
 olive oil pH 8.0, 37°C, recombinant mutant G311D Staphylococcus xylosus
3.1.1.3 50
-
 olive oil pH 8.0, 37°C, recombinant mutant G311L Staphylococcus xylosus
3.1.1.3 75.8
-
 tributyrin pH 8.0, 37°C, recombinant mutant G311D Staphylococcus xylosus
3.1.1.3 250
-
 tributyrin pH 8.0, 37°C, recombinant mutant G311L Staphylococcus xylosus
3.1.1.3 500
-
 olive oil pH 8.0, 37°C, recombinant wild-type enzyme Staphylococcus xylosus
3.1.1.3 1500
-
 tributyrin pH 8.0, 37°C, recombinant wild-type enzyme Staphylococcus xylosus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.3 6.5
-
 mutant G311D Staphylococcus xylosus
3.1.1.3 8
-
 wild-type enzyme Staphylococcus xylosus
3.1.1.3 9.5
-
 mutant G311K Staphylococcus xylosus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.1.3 5 11
-
 Staphylococcus xylosus