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Literature summary extracted from
- Overexpression, purification and characterization of the Trichoderma atroviride endochitinase, Ech42, in Pichia pastoris (2007), Protein Expr. Purif., 55, 183-188.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.2.1.14 | overexpression in Pichia pastoris using a constitutive expression system. The optimal enzyme kinetic parameters for the recombinant protein were identical to those reported for the enzyme isolated from Trichoderma atroviride | Trichoderma atroviride |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.2.1.14 | 1.9 | - |
colloidal chitin | - |
Trichoderma atroviride |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.14 | 45000 | - |
x * 45000, SDS-PAGE | Trichoderma atroviride |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.1.14 | Trichoderma atroviride | - |
- |
- |
Posttranslational Modification
| EC Number | Posttranslational Modification | Comment | Organism |
|---|---|---|---|
| 3.2.1.14 | glycoprotein | - |
Trichoderma atroviride |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.2.1.14 | - |
Trichoderma atroviride |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.2.1.14 | culture medium | - |
Trichoderma atroviride | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.14 | colloidal chitin + H2O | - |
Trichoderma atroviride | ? | - |
? |  |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.2.1.14 | ? | x * 45000, SDS-PAGE | Trichoderma atroviride |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.2.1.14 | Ech42 | - |
Trichoderma atroviride |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.14 | 40 | - |
- |
Trichoderma atroviride |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.14 | 10 | 60 | 10°C: about 50% of maximal activity, 60°C: about 59% of maximal activity | Trichoderma atroviride |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.14 | 4 | - |
- |
Trichoderma atroviride |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.14 | 3 | 7 | pH 3.0: about 35% of maximal activity, pH 7.0: about 60% of maximal activity | Trichoderma atroviride |
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Release 2023.1 (January 2023)
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