Literature summary extracted from

Lunina, N.A.; Agafonova, E.V.; Chekanovskaya, L.A.; Dvortsov, I.A.; Berezina, O.V.; Shedova, E.N.; Kostrov, S.V.; Velikodvorskaya, G.A.
Co-expression of the Thermotoga neapolitana aglB gene with an upstream 3-coding fragment of the malG gene improves enzymatic characteristics of recombinant AglB cyclomaltodextrinase (2007), Protein Expr. Purif., 54, 18-23.

Activating Compound

EC Number	Activating Compound	Comment	Organism	Structure
3.2.1.54	EDTA	at 5 mM for isoenzyme ag1B* and ag1B(1Met), no activity against ag1B(2Met)	Thermotoga neapolitana

Cloned(Commentary)

EC Number	Cloned (Comment)	Organism
3.2.1.54	overexpression in Escherichia coli, three expression vectors are constructed, aglB gene linked to an upstream 90-bp 30-terminal region of the malG gene with the stop codon overlapping with the start codon of aglB, aglB with two tandem potential start codons and ag1B with only one start codon	Thermotoga neapolitana

KM Value [mM]

EC Number	KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
3.2.1.54	0.23	-	p-nitrophenyl-D-maltopentaoside	isoenzyme ag1B*	Thermotoga neapolitana
3.2.1.54	0.27	-	p-nitrophenyl-D-maltopentaoside	isoenzyme ag1B(1Met)	Thermotoga neapolitana
3.2.1.54	1.72	-	p-nitrophenyl-D-maltopentaoside	isoenzyme ag1B(2Met)	Thermotoga neapolitana
3.2.1.54	3.6	-	beta-cyclodextrin	isoenzyme ag1B(1Met)	Thermotoga neapolitana
3.2.1.54	3.9	-	beta-cyclodextrin	isoenzyme ag1B*	Thermotoga neapolitana
3.2.1.54	6.06	-	beta-cyclodextrin	isoenzyme ag1B(2Met)	Thermotoga neapolitana

Molecular Weight [Da]

EC Number	Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
3.2.1.54	55000	-	SDS-PAGE, aglB*	Thermotoga neapolitana

Organism

EC Number	Organism	UniProt	Comment	Textmining
3.2.1.54	Thermotoga neapolitana	-	-	-

Purification (Commentary)

EC Number	Purification (Comment)	Organism
3.2.1.54	by DEAE-Toyopearl chromatography and ultrafiltration	Thermotoga neapolitana

Specific Activity [micromol/min/mg]

EC Number	Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
3.2.1.54	3.1	-	isoenzyme aglB(2Met) after purification, beta-cyclodextrin as substrate	Thermotoga neapolitana
3.2.1.54	22.4	-	isoenzyme aglB(1Met) after purification, beta-cyclodextrin as substrate	Thermotoga neapolitana
3.2.1.54	22.8	-	isoenzyme aglB* after purification, beta-cyclodextrin as substrate	Thermotoga neapolitana

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
3.2.1.54	alpha-cyclodextrin + H2O	-	Thermotoga neapolitana	maltooligosaccharides	maltohexaose, maltoheptaose, maltooctaose	?
3.2.1.54	beta-cyclodextrin + H2O	-	Thermotoga neapolitana	maltooligosaccharides	maltohexaose, maltoheptaose, maltooctaose	?
3.2.1.54	cyclomaltodextrin + H2O	-	Thermotoga neapolitana	linear maltodextrin	-	?
3.2.1.54	gamma-cyclodextrin + H2O	preferred substrate	Thermotoga neapolitana	maltooligosaccharides	maltohexaose, maltoheptaose, maltooctaose	?
3.2.1.54	p-nitrophenyl-D-maltopentaoside + H2O	-	Thermotoga neapolitana	p-nitrophenol + maltopentaose	-	?

Subunits

EC Number	Subunits	Comment	Organism
3.2.1.54	octamer	8 * 55000, aglB*	Thermotoga neapolitana
3.2.1.54	oligomer	aglB(2Met) higher aggregated	Thermotoga neapolitana
3.2.1.54	tetramer	4 * 55000, aglB*	Thermotoga neapolitana

Synonyms

EC Number	Synonyms	Comment	Organism
3.2.1.54	AglB	-	Thermotoga neapolitana
3.2.1.54	CDase	-	Thermotoga neapolitana
3.2.1.54	cyclodextrinase	-	Thermotoga neapolitana
3.2.1.54	cyclomaltodextrinase	-	Thermotoga neapolitana

Temperature Optimum [°C]

EC Number	Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
3.2.1.54	85	-	-	Thermotoga neapolitana

Temperature Stability [°C]

EC Number	Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
3.2.1.54	90	-	half-inactivation time of AglB(2Met) at 90°C is 5 min, for AglB* and AglB(1Met) 3 h	Thermotoga neapolitana

pH Optimum

EC Number	pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
3.2.1.54	6.5	-	-	Thermotoga neapolitana

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)