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Literature summary extracted from

  • Santos, K.; Medrano, F.J.
    Expression, purification, and characterization of an aminopeptidase (Xac2987) with broad specificity from Xanthomonas axonopodis pv. citri (2007), Protein Expr. Purif., 52, 117-122.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.11.20 expressed in Escherichia coli BL21 (DE3) pLysS cells Xanthomonas axonopodis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.11.20 37000
-
 4 * 37000, SDS-PAGE, His-tagged enzyme Xanthomonas axonopodis
3.4.11.20 37250
-
 4 * 37250, dynamic light scattering, His-tagged enzyme Xanthomonas axonopodis
3.4.11.20 148000
-
 His-tagged enzyme, SDS-PAGE Xanthomonas axonopodis
3.4.11.20 149000
-
 His-tagged enzyme, dynamic light scattering Xanthomonas axonopodis

Organism

EC Number Organism UniProt Comment Textmining
3.4.11.20 Xanthomonas axonopodis
-
 Xanthomonas axonopodis citri
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.11.20 Hi-Trap Ni2+-chelating column chromatography Xanthomonas axonopodis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.11.20 Gly-p-nitroanilide + H2O 58.5% activity compared to L-Pro-p-nitroanilide Xanthomonas axonopodis Gly + p-nitroaniline
-
 ?
3.4.11.20 L-Ala-p-nitroanilide + H2O 55.4% activity compared to L-Pro-p-nitroanilide Xanthomonas axonopodis L-Ala + p-nitroaniline
-
 ?
3.4.11.20 L-His-2-naphthylamide + H2O 31.6% activity compared to L-Pro-p-nitroanilide Xanthomonas axonopodis L-His + 2-naphthylamine
-
 ?
3.4.11.20 L-Ile-2-naphthylamide + H2O 4.6% activity compared to L-Pro-p-nitroanilide Xanthomonas axonopodis L-Ile + 2-naphthylamine
-
 ?
3.4.11.20 L-Leu-p-nitroanilide + H2O 8.2% activity compared to L-Pro-p-nitroanilide Xanthomonas axonopodis L-Leu + p-nitroaniline
-
 ?
3.4.11.20 L-Lys-p-nitroanilide + H2O 13% activity compared to L-Pro-p-nitroanilide Xanthomonas axonopodis L-Lys + p-nitroaniline
-
 ?
3.4.11.20 L-Met-p-nitroanilide + H2O 11.5% activity compared to L-Pro-p-nitroanilide Xanthomonas axonopodis L-Met + p-nitroaniline
-
 ?
3.4.11.20 L-Phe-p-nitroanilide + H2O 1.1% activity compared to L-Pro-p-nitroanilide Xanthomonas axonopodis L-Phe + p-nitroaniline
-
 ?
3.4.11.20 L-Pro-2-naphthylamide + H2O 100% activity Xanthomonas axonopodis L-Pro + 2-naphthylamine
-
 ?
3.4.11.20 L-Pro-p-nitroanilide + H2O 100% activity Xanthomonas axonopodis L-Pro + p-nitroaniline
-
 ?
3.4.11.20 L-Ser-2-naphthylamide + H2O 64% activity compared to L-Pro-2-naphthylamide Xanthomonas axonopodis L-Ser + 2-naphthylamine
-
 ?
3.4.11.20 L-Trp-2-naphthylamide + H2O 15.6% activity compared to L-Pro-p-nitroanilide Xanthomonas axonopodis L-Trp + 2-naphthylamine
-
 ?
3.4.11.20 L-Tyr-2-naphthylamide + H2O 30.3% activity compared to L-Pro-p-nitroanilide Xanthomonas axonopodis L-Tyr + 2-naphthylamine
-
 ?
3.4.11.20 L-Val-p-nitroanilide + H2O 5.3% activity compared to L-Pro-p-nitroanilide Xanthomonas axonopodis L-Val + p-nitroaniline
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.11.20 tetramer 4 * 37000, SDS-PAGE, His-tagged enzyme Xanthomonas axonopodis
3.4.11.20 tetramer 4 * 37250, dynamic light scattering, His-tagged enzyme Xanthomonas axonopodis

Synonyms

EC Number Synonyms Comment Organism
3.4.11.20 Xac2987
-
 Xanthomonas axonopodis