Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Van Lanen, S.G.; Lin, S.; Shen, B.
    Biosynthesis of the enediyne antitumor antibiotic C-1027 involves a new branching point in chorismate metabolism (2008), Proc. Natl. Acad. Sci. USA, 105, 494-499.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.3.8.16 cloned into pET-30 Xa/LIC and expressed in Escherichia coli Streptomyces globisporus
2.6.1.86 the gene for SgcD is cloned into pET-30 Xa/LIC and expressed in Escherichia coli Streptomyces globisporus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.3.8.16 0.0012
-
 FMN
-
 Streptomyces globisporus
1.3.8.16 0.056
-
 2-amino-2-deoxyisochorismate
-
 Streptomyces globisporus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.3.8.16 Iron iron-sulfur enzyme. SgcG contains a [Fe-S] cluster with all four Cys found in SgcG involved in iron coordination Streptomyces globisporus
1.3.8.16 Mg2+ activity depends on Streptomyces globisporus
2.6.1.86 Mg2+ SgcD activity is depended on the inclusion of Mg2+ Streptomyces globisporus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.3.8.16 29100
-
 x * 29100, SDS-PAGE Streptomyces globisporus
2.6.1.86 58500
-
 x * 58500, SDS-PAGE Streptomyces globisporus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.3.8.16 2-amino-2-deoxyisochorismate + FMN Streptomyces globisporus the sequential action of 2-amino-4-deoxychorismate synthase (EC 2.6.1.86) and EC 1.3.99.24 leads to the formation of the benzoxazolinate moiety of the enediyne antitumour antibiotic C-1027 3-enolpyruvoylanthranilate + FMNH2
-
 ?
2.6.1.86 chorismate + L-glutamine Streptomyces globisporus the net conversion of chorismate to 3-enolpyruvoylanthranilate by the tandem action of SgcD and SgcG establishes a new branching point in chorismate metabolism leading to synthesis of C-1027, an enediyne antitumor antibiotic (2S)-2-amino-4-deoxychorismate + L-glutamate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.3.8.16 Streptomyces globisporus
-
-
-
2.6.1.86 Streptomyces globisporus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.3.8.16 recombinant enzyme Streptomyces globisporus
2.6.1.86
-
 Streptomyces globisporus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.8.16 2-amino-2-deoxyisochorismate + FMN
-
 Streptomyces globisporus 3-enolpyruvoylanthranilate + FMNH2
-
 ?
1.3.8.16 2-amino-2-deoxyisochorismate + FMN the sequential action of 2-amino-4-deoxychorismate synthase (EC 2.6.1.86) and EC 1.3.99.24 leads to the formation of the benzoxazolinate moiety of the enediyne antitumour antibiotic C-1027 Streptomyces globisporus 3-enolpyruvoylanthranilate + FMNH2
-
 ?
2.6.1.86 chorismate + L-glutamine
-
 Streptomyces globisporus (2S)-2-amino-4-deoxychorismate + L-glutamate
-
 ?
2.6.1.86 chorismate + L-glutamine the net conversion of chorismate to 3-enolpyruvoylanthranilate by the tandem action of SgcD and SgcG establishes a new branching point in chorismate metabolism leading to synthesis of C-1027, an enediyne antitumor antibiotic Streptomyces globisporus (2S)-2-amino-4-deoxychorismate + L-glutamate
-
 ?

Subunits

EC Number Subunits Comment Organism
1.3.8.16 ? x * 29100, SDS-PAGE Streptomyces globisporus
2.6.1.86 ? x * 58500, SDS-PAGE Streptomyces globisporus

Synonyms

EC Number Synonyms Comment Organism
1.3.8.16 ADIC dehydrogenase
-
 Streptomyces globisporus
1.3.8.16 SgcG
-
 Streptomyces globisporus
2.6.1.86 2-amino-2-deoxyisochorismate (ADIC) synthase
-
 Streptomyces globisporus
2.6.1.86 2-amino-2-deoxyisochorismate synthase
-
 Streptomyces globisporus
2.6.1.86 ADIC synthase
-
 Streptomyces globisporus
2.6.1.86 SgcD
-
 Streptomyces globisporus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.3.8.16 15
-
 2-amino-2-deoxyisochorismate
-
 Streptomyces globisporus
1.3.8.16 17
-
 FMN
-
 Streptomyces globisporus

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.8.16 FMN
-
 Streptomyces globisporus