EC Number | Cloned (Comment) | Organism |
---|---|---|
1.3.8.16 | cloned into pET-30 Xa/LIC and expressed in Escherichia coli | Streptomyces globisporus |
2.6.1.86 | the gene for SgcD is cloned into pET-30 Xa/LIC and expressed in Escherichia coli | Streptomyces globisporus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.3.8.16 | 0.0012 | - |
FMN | - |
Streptomyces globisporus | |
1.3.8.16 | 0.056 | - |
2-amino-2-deoxyisochorismate | - |
Streptomyces globisporus |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.3.8.16 | Iron | iron-sulfur enzyme. SgcG contains a [Fe-S] cluster with all four Cys found in SgcG involved in iron coordination | Streptomyces globisporus | |
1.3.8.16 | Mg2+ | activity depends on | Streptomyces globisporus | |
2.6.1.86 | Mg2+ | SgcD activity is depended on the inclusion of Mg2+ | Streptomyces globisporus |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.3.8.16 | 29100 | - |
x * 29100, SDS-PAGE | Streptomyces globisporus |
2.6.1.86 | 58500 | - |
x * 58500, SDS-PAGE | Streptomyces globisporus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.3.8.16 | 2-amino-2-deoxyisochorismate + FMN | Streptomyces globisporus | the sequential action of 2-amino-4-deoxychorismate synthase (EC 2.6.1.86) and EC 1.3.99.24 leads to the formation of the benzoxazolinate moiety of the enediyne antitumour antibiotic C-1027 | 3-enolpyruvoylanthranilate + FMNH2 | - |
? | |
2.6.1.86 | chorismate + L-glutamine | Streptomyces globisporus | the net conversion of chorismate to 3-enolpyruvoylanthranilate by the tandem action of SgcD and SgcG establishes a new branching point in chorismate metabolism leading to synthesis of C-1027, an enediyne antitumor antibiotic | (2S)-2-amino-4-deoxychorismate + L-glutamate | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.3.8.16 | Streptomyces globisporus | - |
- |
- |
2.6.1.86 | Streptomyces globisporus | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.3.8.16 | recombinant enzyme | Streptomyces globisporus |
2.6.1.86 | - |
Streptomyces globisporus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.3.8.16 | 2-amino-2-deoxyisochorismate + FMN | - |
Streptomyces globisporus | 3-enolpyruvoylanthranilate + FMNH2 | - |
? | |
1.3.8.16 | 2-amino-2-deoxyisochorismate + FMN | the sequential action of 2-amino-4-deoxychorismate synthase (EC 2.6.1.86) and EC 1.3.99.24 leads to the formation of the benzoxazolinate moiety of the enediyne antitumour antibiotic C-1027 | Streptomyces globisporus | 3-enolpyruvoylanthranilate + FMNH2 | - |
? | |
2.6.1.86 | chorismate + L-glutamine | - |
Streptomyces globisporus | (2S)-2-amino-4-deoxychorismate + L-glutamate | - |
? | |
2.6.1.86 | chorismate + L-glutamine | the net conversion of chorismate to 3-enolpyruvoylanthranilate by the tandem action of SgcD and SgcG establishes a new branching point in chorismate metabolism leading to synthesis of C-1027, an enediyne antitumor antibiotic | Streptomyces globisporus | (2S)-2-amino-4-deoxychorismate + L-glutamate | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.3.8.16 | ? | x * 29100, SDS-PAGE | Streptomyces globisporus |
2.6.1.86 | ? | x * 58500, SDS-PAGE | Streptomyces globisporus |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.3.8.16 | ADIC dehydrogenase | - |
Streptomyces globisporus |
1.3.8.16 | SgcG | - |
Streptomyces globisporus |
2.6.1.86 | 2-amino-2-deoxyisochorismate (ADIC) synthase | - |
Streptomyces globisporus |
2.6.1.86 | 2-amino-2-deoxyisochorismate synthase | - |
Streptomyces globisporus |
2.6.1.86 | ADIC synthase | - |
Streptomyces globisporus |
2.6.1.86 | SgcD | - |
Streptomyces globisporus |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.3.8.16 | 15 | - |
2-amino-2-deoxyisochorismate | - |
Streptomyces globisporus | |
1.3.8.16 | 17 | - |
FMN | - |
Streptomyces globisporus |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.3.8.16 | FMN | - |
Streptomyces globisporus |