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Literature summary extracted from
- The 39-kDa poly(ADP-ribose) glycohydrolase ARH3 hydrolyzes O-acetyl-ADP-ribose, a product of the Sir2 family of acetyl-histone deacetylases (2006), Proc. Natl. Acad. Sci. USA, 103, 16687-16691.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.2.2.19 | - |
Homo sapiens |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 3.2.1.143 | D77N/D78N | mutation abolishes the hydrolytic activity on O-acetyl-ADP-ribose | Homo sapiens |
| 3.2.2.19 | D77/78N | mutant does not hydrolyze poly(ADP-ribose) | Homo sapiens |
| 3.2.2.19 | E238/239Q | mutant hydrolyzes O-acetyl-ADP-ribose at rates similar to that of the wild-type | Homo sapiens |
| 3.2.2.19 | E261/262Q | mutant hydrolyzes O-acetyl-ADP-ribose at rates similar to that of the wild-type | Homo sapiens |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.2.2.19 | ADP-ribose | - |
Homo sapiens |  |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.2.1.143 | Mg2+ | required for hydrolysis of O-acetyl-ADP-ribose | Homo sapiens | |
| 3.2.2.19 | Mg2+ | - |
Homo sapiens | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.2.2.19 | 39000 | - |
- |
Homo sapiens |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.2.19 | 2'-O-acetyl-ADP-ribose + H2O | Homo sapiens | - |
ADP-ribose + acetate | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.1.143 | Homo sapiens | - |
isoform ARH3 | - |
| 3.2.2.19 | Homo sapiens | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.2.2.19 | - |
Homo sapiens |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.143 | 2'-O-acetyl-ADP-ribose + H2O | rate of hydrolysis by isoform ARH3 is 250fold that observed with isoform ARH1, isoform ARH2 is inactive with this substrate | Homo sapiens | ADP-ribose + acetate | - |
? | |
| 3.2.2.19 | 2'-O-acetyl-ADP-ribose + H2O | - |
Homo sapiens | ADP-ribose + acetate | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.2.2.19 | ARH3 | - |
Homo sapiens |
| 3.2.2.19 | O-acetyl-ADP-ribose hydrolase | - |
Homo sapiens |
| 3.2.2.19 | poly-(ADP-ribose) glycohydrolase | - |
Homo sapiens |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.2.19 | 7 | - |
hydrolysis of O-acetyl-[14C]ADP-ribose catalyzed by ARH3 | Homo sapiens |
Ki Value [mM]
| EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.2.2.19 | 0.002 | - |
ADP-ribose | competitive inhibition | Homo sapiens | |
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Release 2023.1 (January 2023)
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