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Literature summary extracted from

  • Miranda, J.A.; Avonce, N.; Suarez, R.; Thevelein, J.M.; Van Dijck, P.; Iturriaga, G.
    A bifunctional TPS-TPP enzyme from yeast confers tolerance to multiple and extreme abiotic-stress conditions in transgenic Arabidopsis (2007), Planta, 226, 1411-1421.
    View publication on PubMed

Application

EC Number Application Comment Organism
2.4.1.15 agriculture the results demonstrate that engineering trehalose metabolism with a yeast TPS-TPP bifunctional enzyme confers multiple stress protection in plants, comprising a potential tool to improve stress-tolerance in crops Saccharomyces cerevisiae

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.15 a translational trehalose-6-phosphate synthase - trehalose-6-phosphate phosphatase, TPS-TPP, gene-fusion is constructed and subcloned into the pBluescript SK vector, into the yeast expression vector pSal4, and into the pBin19 vector for transformation of Arabidopsis thaliana Saccharomyces cerevisiae
3.1.3.12 expressed in Arabidopsis thaliana Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.15 UDP-alpha-D-glucose + D-glucose 6-phosphate Saccharomyces cerevisiae
-
 UDP + alpha,alpha-trehalose 6-phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.15 Saccharomyces cerevisiae
-
-
-
3.1.3.12 Saccharomyces cerevisiae
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.15 0.011
-
 TPS specific activity in glucose, yeast mutant strain tps2delta + pSAL4 Saccharomyces cerevisiae
2.4.1.15 0.013
-
 TPS specific activity in galactose, yeast mutant strain tps1delta_tps2delta + pSAL4::TPS1 Saccharomyces cerevisiae
2.4.1.15 0.02
-
 TPS specific activity in glucose, yeast mutant strain tps1delta_tps2delta + pSAL4::TPS1 Saccharomyces cerevisiae
2.4.1.15 0.055
-
 TPS specific activity in galactose, yeast wild type + pSAL4 Saccharomyces cerevisiae
2.4.1.15 0.063
-
 TPS specific activity in galactose, yeast mutant strain tps2delta + pSAL4::TPS2 Saccharomyces cerevisiae
2.4.1.15 0.067
-
 TPS specific activity in glucose, yeast mutant strain tps2delta + pSAL4::TPS2 Saccharomyces cerevisiae
2.4.1.15 0.073
-
 TPS specific activity in glucose, yeast mutant strain tps2delta + pSAL4::TPS1-TPS2 Saccharomyces cerevisiae
2.4.1.15 0.078
-
 TPS specific activity in galactose, yeast mutant strain tps2delta + pSAL4::TPS1-TPS2 Saccharomyces cerevisiae
2.4.1.15 0.079
-
 TPS specific activity in glucose, yeast wild type + pSAL4 Saccharomyces cerevisiae
2.4.1.15 0.081
-
 TPS specific activity in galactose, yeast mutant strain tps1delta + pSAL4::TPS1-TPS2 Saccharomyces cerevisiae
2.4.1.15 0.086
-
 TPS specific activity in glucose, yeast mutant strain tps1delta + pSAL4::TPS1-TPS2 Saccharomyces cerevisiae
2.4.1.15 0.088
-
 TPS specific activity in galactose, yeast mutant strain tps1delta_tps2delta + pSAL4::TPS1-TPS2 Saccharomyces cerevisiae
2.4.1.15 0.093
-
 TPS specific activity in glucose, yeast mutant strain tps1delta_tps2delta + pSAL4::TPS1-TPS2 Saccharomyces cerevisiae
2.4.1.15 0.144
-
 TPS specific activity in glucose, yeast mutant strain tps1delta + pSAL4::TPS1 Saccharomyces cerevisiae
2.4.1.15 0.163
-
 TPS specific activity in galactose, yeast mutant strain tps1delta + pSAL4::TPS1 Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.15 UDP-alpha-D-glucose + D-glucose 6-phosphate
-
 Saccharomyces cerevisiae UDP + alpha,alpha-trehalose 6-phosphate
-
 ?
3.1.3.12 trehalose 6-phosphate + H2O
-
 Saccharomyces cerevisiae trehalose + phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.15 TPS1
-
 Saccharomyces cerevisiae
2.4.1.15 trehalose-6-phosphate synthase
-
 Saccharomyces cerevisiae
3.1.3.12 TPP
-
 Saccharomyces cerevisiae
3.1.3.12 TPS2
-
 Saccharomyces cerevisiae
3.1.3.12 trehalose-6-phosphate phosphatase
-
 Saccharomyces cerevisiae